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Glucosyltransferases (Gtfs) play a crucial role in the pathogenesis of dental caries caused by Streptococcus mutans. Gtfs is recognized as an attractive target for effective therapeutic interventions aimed at inhibiting cariogenic biofilms.
Objective: This study evaluates the antibacterial effects of sage extract compared to chlorhexidine mouthwashes on the expression of Gtf genes in S. mutans from salivary samples of high caries risk patients over a one-month follow-up, utilizing real-time polymerase chain reaction.
Methodology: This clinical trial involves 42 participants with a high risk for caries, who are randomly divided into two groups of 21 subjects each.
Glucosyltransferases (Gtfs) are essential in the development of dental caries caused by Streptococcus mutans. They are currently recognized as promising targets for effective therapeutic strategies aimed at disrupting cariogenic biofilms.
Objective: This study investigates the antibacterial effects of sage extract in comparison to chlorhexidine mouthwashes on the expression of Gtf genes in S. mutans from the saliva of patients at high risk for caries, over a one-month follow-up period, using real-time polymerase chain reaction.
Methodology: This clinical trial includes 42 high caries risk participants, who are randomly assigned to two groups, each consisting of 21 subjects.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sage oil mouthwash | Experimental | Sage oil obtained from a local market of herbal products in Egypt. In order to identify chemical composition of sage essential oils and overall purity, Gas Chromatography/ Mass Spectrometry analysis (GC/MS) performed |
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| Chlorohexidine Mouthwash | Active Comparator | 0.12% chlorhexidine gluconate in a base containing water, 11.6% alcohol, glycerin, PEG-40 sorbitan diisostearate, flavor, and sodium saccharin |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sage Oil Mouthwash | Other | Sage oil obtained from a local market of herbal products in Egypt. In order to identify chemical composition of sage essential oils and overall purity, Gas Chromatography/ Mass Spectrometry analysis (GC/MS) performed |
| Measure | Description | Time Frame |
|---|---|---|
| real-time PCR | S. Mutans, Gtf B, Gtf D | 1 week |
| real-time PCR | S. Mutans, Gtf B, Gtf D | 2 weeks |
| real-time PCR | S. Mutans, Gtf B, Gtf D | 3 weeks |
| real-time PCR | S. Mutans, Gtf B, Gtf D | 4 weeks |
| Gene Expression | S. Mutans, Gene Expression | 1 week |
| Gene Expression | S. Mutans, Gene Expression | 2 weeks |
| Gene Expression | S. Mutans, Gene Expression | 3 weeks |
| Gene Expression | S. Mutans, Gene Expression | 4 weeks |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Cairo University | Cairo | Egypt |
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| ID | Term |
|---|---|
| D003731 | Dental Caries |
| D009057 | Stomatognathic Diseases |
| ID | Term |
|---|---|
| D017001 | Tooth Demineralization |
| D014076 | Tooth Diseases |
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| ID | Term |
|---|---|
| D001688 | Biological Products |
| ID | Term |
|---|---|
| D045424 | Complex Mixtures |
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Sage Extract Natural product
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same bottles dark in color was used
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| Chlorohexidine Mouthwash | Biological | 0.12% chlorhexidine gluconate in a base containing water, 11.6% alcohol, glycerin, PEG-40 sorbitan diisostearate, flavor, and sodium saccharin |
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