Spinal Cord Stimulation (SCS) is a neuromodulation that involves the implantation of a device that delivers electrical impulses to the spinal cord through an electrode placed in the epidural space. The stimulation alters pain signals before they reach the brain, effectively masking or reducing the sensation of pain.

Dorsal Root Ganglion Stimulation (DRGS) is a targeted neuromodulation technique for managing chronic pain. The procedure begins with a thorough preoperative assessment, including imaging to locate the specific dorsal root ganglion (DRG) associated with the pain. Under local anesthesia, a small electrode is implanted near the DRG. This is done through a minimally invasive procedure, often guided by fluoroscopy or CT imaging to ensure precise placement.

Motor Cortex Stimulation (MCS) is a neuromodulation technique that involves the surgical implantation of electrodes over the motor cortex, typically targeting the precentral gyrus, to deliver electrical stimulation. The procedure involves placing an electrode grid or strip on the dura mater overlying the motor cortex, which is identified via neuroimaging techniques such as functional MRI or neuronavigation. Once implanted, the electrodes are connected to an implanted pulse generator (IPG), which delivers adjustable electrical impulses.

Self-report questionnaires where participants rate their sense of agency over the prosthesis during tasks. It uses 0 to 10 scale, where 0 means lower agency and 10 means higher agency

Throughout the study, participants will be asked to complete questionnaires assessing the degree of embodiment of the prosthesis. Since during the experimental sessions, feedback from the prosthesis is projected onto the phantom limb, the research team anticipates observing a transfer effect from the phantom limb to the robotic hand. The degree of this transfer will be evaluated using the Embodiment questionnaires for both upper and lower limbs.

Embodiment uses scale from -9 to 9; -9 means "No embodiment" and 9 means "High embodiment".

The questionnaire is intended for completion by a physician and combines a pain distribution diagram with a visual analog scale (VAS) and a section focused on identifying spontaneous and triggered neuropathic pain symptoms. It also assesses the nature of pain using the diagram, categorizing it as constant, episodic, or constant with episodes, among others. The questionnaire comprehensively reflects all possible pain parameters, allowing for clear tracking of pain dynamics over time.

The total score ranges from -1 to 38. Based on this score:

≤ 12: Neuropathic pain is unlikely. 13-18: The result is ambiguous; further assessment may be needed.

≥ 19: Neuropathic pain is likely.

The Hospital Anxiety and Depression Scale (HADS) is a widely used screening tool designed to assess anxiety and depression levels in patients in non-psychiatric hospital settings. It consists of 14 items, divided into two subscales: seven questions assess anxiety (HADS-A) and seven assess depression (HADS-D). The items focus on emotional and cognitive symptoms rather than physical symptoms, making it suitable for patients with medical conditions. Each item is scored on a scale of 0 to 3, with total scores for each subscale ranging from 0 to 21. A score of 8 or more on either subscale suggests the presence of anxiety or depression. The HADS is valued for its simplicity and reliability in clinical and research settings.

Self-report questionnaires where participants rate their sense of ownership over the prosthesis during tasks. It uses 0 to 10 scale, where 0 means lower agency and 10 means higher agency

Value of sensory discrimination is measured as Accuracy of Object Recognition. In this task, patients distinguish object sizes using feedback from stimulation through a prosthetic hand. The hand grasps objects made from various materials, with different shapes, or the same shape but different diameters. Concurrently, signals from the prosthetic sensors are translated into patterns of electrical stimulation. When the patient initiates a grasp, changes in the sensor signals result in adjustments to the amplitude of the neurostimulation. The patient's goal is to identify the properties of the objects based solely on the neurostimulation signals, while visual and auditory cues are blocked with a mask and headphones. It is expected that as patients adapt to the prosthetic system, their performance in recognizing objects will improve over time.

In this task, the participant is presented with three identical objects, each assigned to one of three classes: (1) a non-tactile object, (2) a tactile object, and (3) a highly tactile object. At the start of each trial, the object classes are randomly reassigned. The distinction between the classes is that, upon touching an object of class (1) with the prosthesis, the participant receives no feedback; touching a class (2) object triggers low-amplitude stimulation, and a class (3) object triggers high-amplitude stimulation. During the trial, the participant uses the prosthesis to examine each object and determine which one belongs to class 2. As a final measurement investigators take the accuracy in this task.

In a task requiring the discrimination of objects using feedback from stimulation, the prosthetic hand grasps objects made of different materials, varying shapes, or identical shapes but different diameters. Simultaneously, signals from the prosthetic sensors are transformed into patterns of electrical stimulation. When the patient initiates a grasp, changes in the sensor data result in alterations in the amplitude of neurostimulation. The patient's task is to determine the properties of the objects based on the signals from neurostimulation, while other sources of information-visual and auditory-are blocked using a bandage and headphones. It is expected that over time, as patients adapt to the prosthetic system, their performance in this task will improve. The final measure is an accuracy of discrimination computed as correct responses divided bu number of trials

The test requires at least two movements in the prosthesis: hand opening-closing and wrist supination-pronation. In the test, the subject stands with the prosthetic arm lowered and elbow extended. They must move three red clips from a horizontal to a vertical rod and back, using a Rolyan Graded Pinch Exerciser. The feet must remain on the floor, and the time to complete the task is recorded.

In this task, the participant is asked to move a fragile object from one area to another using a neuroprosthetic system. The challenge lies in the fact that moving the fragile object requires precise motor control, as excessive pressure may break it. The participant must move the object as many times as possible within a 1-minute window.

In this paradigm, the participant controls a prosthesis in VR. Myographic sensors control various grasps of the virtual prosthesis, and when the prosthetic avatar touches virtual objects, a neurostimulation-based feedback system is activated. The quality of prosthesis control is estimated

In this task, the participant takes a fixed-duration walk on a treadmill at a set speed while connected to a motion capture system and spirometry. The walk includes two modes: with and without feedback. The leg prosthesis feedback system operates via signals from a smart insole, modulating stimulation based on signal changes. Gait patterns from sessions with and without feedback will be compared, and changes in respiratory patterns are also expected between the two modes.

In this task, a smart insole equipped with a pressure sensor system is connected to the leg prosthesis. Using transformation of the sensor data, the neurostimulation signal is modulated to encode different patterns of pressure applied to the insole. The patient's objective is to learn to distinguish between various surfaces in contact with the insole. This test assesses the sensory discrimination capabilities of the sensory-enabled lower limb prosthesis.

The test aims to compare functional mobility when ascending and descending stairs with and without feedback. The time taken to climb one flight of stairs, turn on the landing, and descend is measured. Multiple attempts are given with a 2-minute rest between them, and the best result is recorded.

OPUS (Optimal Patient Utilization System) is a clinical decision support tool designed to enhance patient care through improved resource management and treatment efficiency. It utilizes advanced algorithms and data analytics to optimize patient scheduling, treatment pathways, and staff allocation within healthcare facilities. By analyzing patient demographics, medical history, and treatment outcomes, OPUS helps clinicians make informed decisions about resource allocation and care delivery. The system aims to reduce wait times, streamline operations, and improve overall patient satisfaction. Additionally, OPUS provides real-time feedback and reporting capabilities, enabling healthcare providers to monitor performance metrics and identify areas for improvement, ultimately enhancing the quality of care.

The Prosthesis Evaluation Questionnaire (PEQ) is a standardized assessment tool designed to evaluate the functional and psychosocial outcomes of individuals using prosthetic devices. Comprising various domains, the PEQ addresses aspects such as mobility, activity level, and quality of life, allowing users to report their experiences and satisfaction with their prosthesis. The questionnaire includes specific items that gauge the user's perception of their prosthetic limb's comfort, functionality, and impact on daily activities. PEQ ranges from 0 to 100: 0 indicates the worst possible outcome (e.g., "not at all satisfied") and 100 indicates the best possible outcome (e.g., "completely satisfied").

The setup includes an open box with a divider, 150 wooden blocks, and a timer. The participant, seated at a table, has 60 seconds to move as many blocks as possible, one at a time, over the divider using one hand.

To assess the functional state of motor nerves in the amputated limb, transcranial magnetic stimulation (TMS) will be used. A TMS stimulator, consisting of one or two electromagnetic coils, will be placed over the motor cortex, while an electromyographic (EMG) electrode will be positioned over the target muscle of the amputated limb. A current pulse through the TMS coils will induce currents in the motor cortex, activating it and eliciting motor-evoked potentials (MEPs) in the target muscle. Delays and EMG potential profiles will inform on motor nerve function and the suitability of neuromodulation, as well as the therapeutic effects of TMS for phantom limb pain relief.