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To investigate the influence of Neuromediators expression in corneal stromal lenticules and corneal injury repair after small incision extraction
This study included 160 eyes from high myopia patients who underwent Small Incision Lenticule Extraction. Patients were devided into three groups based on duration of wearing SCLs: > 10 years group, ≤ 10 years group, and NW group (not SCLs). Corneal higher-order aberrations and densitometry were evaluated using a Pentacam system. Neuromediator levels in corneal stromal lenticules were analyzed using ELISA and immunofluorescence
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| SCLs >10 years | high myopia patients who wear SCL more than 10 years before Small Incision Lenticule Extraction |
| |
| SCLs ≤10 years | high myopia patients who wear SCL for 10 years or less before Small Incision Lenticule Extraction |
| |
| NW | high myopia patients who have not worn SCLs before Small Incision Lenticule Extraction |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| This study does not involve any interventions | Other | This study does not involve any interventions |
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| Measure | Description | Time Frame |
|---|---|---|
| Corneal stromal lenticule Neuromediators | Corneal neuromediators, including NGF(pg/mL), CGRP(pg/mL), SP(pg/mL), IGFBP-1(ng/mL), and MANF(pg/mL), which play pivotal roles in maintaining normal physiological functions of the cornea. | baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Corneal Aberration | Preoperative corneal aberration measurements were performed using a Scheimpflug tomography system (Pentacam; Oculus GmbH, Wetzlar, Germany). Images of the highest quality (designated as OK) were selected for analysis. The coefficients for a standardized diameter of 8 ?mm were examined, followed by an assessment of the clinical significance of total higher-order aberrations (HOA, mm), spherical aberration (Z4, 0, mm), horizontal coma (Z3, 1, mm), vertical coma (Z3, -1, mm), horizontal trefoil (Z3, 3, mm), and oblique trefoil (Z3, -3, mm) in relation to visual quality. |
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Inclusion Criteria:
Exclusion Criteria:
- a history of moderate to severe dry eye as well as any ocular or systemic disease that would contraindicate laser refractive surgery.
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patients with high myopia who underwent SMILE surgery at Shanghai Tenth People's Hospital in Shanghai, China, between October 2020 and October 2023.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| min Li, Dr | Contact | +86 18817367841 | rgswan@163.com | |
| Yuhao Shao, MD | Contact |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Shanghai Tenth People's Hospital | Recruiting | Shanghai | Shanghai Municipality | China |
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All samples were collected from participants with high myopia who underwent SMILE surgery. The VisuMax femtosecond laser system (Carl Zeiss Meditec, Jena, Germany) was utilized with the following settings: a repetition rate of 500 kHz, pulse energy of 150 nJ, intended cap thickness of 110-120 μm, and optical zone of 6.0-6.8 mm. A circumferential incision width of 2 mm was made for lenticule dissection and extraction. The surgical procedures were performed by a single experienced surgeon (Dr. Zou). Following the SMILE surgeries, corneal stromal lenticules were transferred to Eppendorf tubes and stored at -80°C. for subsequent analysis.
| baseline |
| Corneal Densitometry | The Pentacam HR was used to collect data on corneal densitometry values, which were expressed in grayscale units (GSUs), playing a crucial role in quantifying corneal clarity by monitoring transient haze-like reactions. The cornea was divided into four annular zones (0-2 mm, 2-6 mm, 6-10 mm, and 10-12 mm), each exhibiting distinct corneal densitometry values. Based on the anatomical layers of the cornea at different depths, it consists of four layers: a superficial anterior layer of 120 μm, a posterior layer located within the innermost part of the cornea measuring 60 μm; central layers calculated by subtracting thickness of the anterior and posterior layers from the total layer thickness; and a total layer. Corneal densitometry values were measured in the 0-2 mm and 2-6 mm to correspond to the corneal optical zone diameter of SMILE. | baseline |