This paper is centrifuged and your tears are collected then frozen before analysis. We collect the fat from your tears by scraping the edge of your lower eyelid with small sterile tweezers. All surgical waste will be collected during eye surgeries only if you must have them.

During each ocular surgical procedure, the vitreous, subretinal fluids and/or aqueous humor will eventually be recovered when required by the procedure. The rest of the blood samples, not used during diagnostic analyses, will be kept for later analyses.

Tears will be collected using a non-invasive and painless method that takes around ten minutes. The tissues (connectiva, cornea, retina, epi-retinal membrane) which must have been removed when required by the surgical procedure will also be preserved.

• OSDI score
• QoL score VF24 questionnaire
• Stage and type of Rosacea
• Blepharitis stage
• Oxford score
• Schirmer score
• OSI Index
• Meibomian meibography score
• Quantification of corneal opacity and neovascularization by standardized score
• Limbal insufficiency score
• Clinical evolution under therapeutic effect

• Measurement of total choroidal thickness
• Measurement of choroidal vascular caliber
• Measurement of foveolar avascular area
• Calculation of fundus autofluorescence areas
• Calculation of retinal non-perfusion areas
• Cone counting

Progression of limbic insufficiency (Deng grades) of ocular rosacea;

• Ocular dryness (Oxford score, OSDI questionnaire);
• Quantification of corneal nerves (density),
• tear film (OSI score, Schirmer score, BUT score, OXFORD score),
• meibomian glands (meibographic score);
• variation in the thickness of the subfoveal choroid, a vascular index of the choroid quantified on analysis of the foveolar section in EDI;
• Quantification of the surface area of epithelial atrophy on blue autofluorescence blue ;