The pairing of high-effort exercise, called conditioning activity, with a biomechanically similar high-velocity power movement is considered a valid method to effectively facilitate muscle power adaptations. Research has mainly focused on using this method in exercises performed by both limbs, even though sports tasks are often executed through motion sequences produced by a single limb alternately. However, available studies have focused solely on the dominant leg, leaving it unclear if the effectiveness is similar in both legs and how it transfers between them. Therefore, this study aims to evaluate the immediate effects of the isometric split squat exercise on single-leg drop jump performance in volleyball players, considering their training status.

Inclusion Criteria:

• no lower-limb serious injury, including tendon or muscle tear,
• participation in regular resistance training and competition.
• for the elite group participants competed at the highest national volleyball league for at least two consecutive seasons.
• for the amateur group participants competed in junior groups outside the highest league level.

Exclusion Criteria:

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