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| Name | Class |
|---|---|
| Research Foundation Flanders | OTHER |
| Ziekenhuis Oost-Limburg | OTHER |
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Our society is characterized by a strong increase in the prevalence of obesity, which often causes the development of cardiovascular and metabolic diseases such as type 2 diabetes. The way(s) obesity is responsible for these diseases, is still insufficiently understood. This study therefore examines the content of human fat tissue storage location- and cell type-specific extracellular vesicles (EVs) in lean and obese individuals, and the possible connection with sex, insulin sensitivity, and the blood-brain barrier.
This study focuses on unraveling the fundamental differences in communication via extracellular vesicles (EVs) derived from fat cells and immune cells in terms of fat tissue storage location and sex among individuals with and without obesity. The study hypothesizes that the differences in EV profiles, originating from corresponding in vitro differentiated hMADS and ex vivo isolated adult fat cells and immune cells, among individuals with and without obesity can be related to sex and/or fat tissue storage location. Additionally, the effect of the isolated EVs on the blood-brain barrier function is also investigated.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| EVO participants with obesity | From each patient, two adipose tissue biopsies will be taken during bariatric surgery; one from the subcutaneous and one from the visceral adipose tissue. |
| |
| EVO participants without obesity | From each lean patient, two adipose tissue biopsies will be taken during abdominal surgery; one from the subcutaneous and one from the visceral adipose tissue. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Adipose tissue biopsy | Other | From each patient, two biopsies will be taken during surgery; one from the subcutaneous and one from the visceral adipose tissue. |
|
| Measure | Description | Time Frame |
|---|---|---|
| General characterization of adipocyte- or adipose tissue-derived extracellular vesicles | Concentration, size, surface markers, content and morphology of extracellular vesicles derived from isolated mature adipocytes, isolated adipose tissue stem cells or isolated from complete adipose tissue explants derived from both the subcutaneous as the visceral adipose tissue biopsies will be investigated. | Baseline |
| General characterization of adipose tissue- and blood immune cells | Immune cells isolated from adipose tissue and blood will be characterized by flow cytometry. | Baseline |
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| Measure | Description | Time Frame |
|---|---|---|
| Correlations between possible influencing patient factors (derived by a questionnaire) and extracellular vesicle characteristics | Questions about general health (physical activity, sedentary behavior, sleeping pattern, ...), physical condition before surgery (nausea, food intake, ...), etc. This information will be used to make correlations with extracellular vesicles characteristics. | Baseline |
Individuals with obesity - Inclusion Criteria:
Individuals with obesity - Exclusion Criteria:
Individuals without obesity (lean) - inclusion criteria
Individuals without obesity (lean) - exclusion criteria
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Individuals with or without obesity presenting at the bariatric surgery or abdominal surgery department, respectively.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Lisa Mennens | Contact | +32 (0)499 / 72 29 93 | lisa.mennens@uhasselt.be | |
| Kenneth Verboven | Contact | +32(0)11 26 93 15 | kenneth.verboven@uhasselt.be |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ziekenhuis Oost-Limburg | Recruiting | Genk | Limburg | 3600 | Belgium |
Patient information will be coded based on unique identifiers. This file will only be available for the principle investigator and will be stored safely on the secured Google Enterprise drive of UHasselt. Biological samples will be processed and coded by UHasselt, after registration in the biobank of Limburg (UBILIM). Only researchers of the EVO study will have access to any of the files, patient information or biological samples.
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| ID | Term |
|---|---|
| D009765 | Obesity |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| D011795 | Surveys and Questionnaires |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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peripheral blood + subcutaneous/visceral adipose tissue biopsies
| Blood sampling | Other | From each patient, two venous blood samples (citrate, LiHe and EDTA plasma) will be taken before surgery. |
|
| Questionnaire | Other | Before bariatric surgery, every patient will fill out questions about general health (physical activity, sedentary behavior, sleeping pattern, ...), physical condition before surgery (nausea, food intake, ...), etc. |
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| Patient phenotyping | Other | Length and weight will be measured to calculate body mass index (BMI). Waist and hip circumference will be assessed, as well as blood pressure (both systolic and diastolic) and heart rate at rest. Body composition will be determined using a bioelectrical impedance analysis (BIA) in the hospital. Medication use and comorbidities (that are allowed within the in-/exclusion criteria) will also be derived from the patient's medical files with patient's permission. |
|
| Correlations between possible influencing patient factors (derived by patient phenotyping) and extracellular vesicle characteristics | Length and weight will be measured to calculate body mass index (BMI). Waist and hip circumference will be assessed, as well as blood pressure (both systolic and diastolic) and heart rate at rest. Body composition will be determined using a bioelectrical impedance analysis (BIA). Medicine use and comorbidities (that are allowed within the in-/exclusion criteria) will also be derived from the patient's medical files with patient's permission. This information will be used to make correlations with extracellular vesicles characteristics. | Baseline |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |
| D003625 | Data Collection |
| D004812 | Epidemiologic Methods |
| D017531 | Health Care Evaluation Mechanisms |
| D011787 | Quality of Health Care |
| D017530 | Health Care Quality, Access, and Evaluation |
| D011634 | Public Health |
| D004778 | Environment and Public Health |