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The aim of the research project was to investigate the effects of hyperbaric oxygen (HBO) treatment on the redox equilibrium in individuals with different pathological conditions. Both hyperbaric oxygenation and the pathological condition are associated with redox imbalance (oxidative stress), however, HBO is successfully used in the treatment of chronic wounds, e.g., diabetic foot syndrome, as well as in sudden and acute hearing loss, e.g., acute acoustic trauma or idiopathic sudden sensorineural hearing loss.
The study involed in sudden sensorineural hearing loss (SSNHL) patients, individuals with difficult-to-heal skin wounds following mechanical injuries, and diabetic foot patients. HBO therapy, which involved inhalation of 100% oxygen at a pressure of 2.5 atm, consisted of 14-30 sessions depending on the clinical case. The study material was venous blood drawn three times: before and after the first session, and after the last HBO procedure. The following variables were evaluated: the concentrations of thiobarbituric acid reactive substances (TBARS), malondialdehyde (MDA) and conjugated dienes (CD) in plasma and erythrocytes, the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in erythrocytes, as well as, blood counts.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Patients | Experimental | Hyperbaric oxygen (HBO) therapy. |
|
| Controls | No Intervention | Healthy individuals that never treated with HBO therapy. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Hyperbaric Oxygen Therapy | Procedure | HBO session includes three 20-minute inhalations of pure oxygen in a hyperbaric chamber utilizing specially fitted face masks for each patient. The session is conducted as follows: 10 minutes compression of up to 2.5 atm (0.25 MPa), inhalation of 100% oxygen for 20 minutes, a 5-minute break in the chamber without the mask (oxygen concentration up to 23%, constant temperature (room) and humidity), again 20 minutes inhalation of 100% oxygen, another 5-minute break (the same condition), the last 20 minutes inhalation of 100% oxygen, and finally a 10-minute decompression. The total duration of the session was 90 minutes. |
| Measure | Description | Time Frame |
|---|---|---|
| Thiobarbituric acid reactive substances (TBARS) | Lipid peroxidation index represented by malondialdehyde (MDA), determined in plasma and erythrocytes. | Three days, three different condition measurements |
| Malondialdehyde (MDA) | Lipid peroxidation index; pure MDA determined using high-performance liquid chromatography in plasma and erythrocytes. | Three days, three different condition measurements |
| Conjugated dienes (CD) | Lipid peroxidation index, determined in plasma and erythrocytes. | Three days, three different condition measurements |
| Catalase (CAT) | Antioxidant enzyme, determined in erythrocytes. | Three days, three different condition measurements |
| Superoxide dismutase (SOD) | Antioxidant enzyme, determined in erythrocytes. | Three days, three different condition measurements |
| Glutathione peroxidase (GPx) | Antioxidant enzyme, determined in erythrocytes. | Three days, three different condition measurements |
| Measure | Description | Time Frame |
|---|---|---|
| White blood cell count (WBC) | Leukocytes found in peripheral blood with specyfication of their types: lymphocytes, monocytes, neutrophils, eosinophils, basophils. | Two days, two different condition measurements |
| Red blood cell count (RBS) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Paweł Sutkowy, PhD | Department of Medical Biology and Biochemistry, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Karlowicza 24, 85-092 Bydgoszcz, Poland | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Medical Biology and Biochemistry, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Karlowicza 24, 85-092 Bydgoszcz, Poland | Bydgoszcz | Kuyavian-Pomeranian Voivodeship | 85-092 | Poland |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 39425927 | Derived | Sutkowy P, Paprocki J, Piechocki J, Wozniak A. The impact of hyperbaric oxygen therapy on the redox balance of patients with diabetic foot syndrome. Free Radic Res. 2024 Nov;58(11):723-732. doi: 10.1080/10715762.2024.2417286. Epub 2024 Oct 19. |
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There is no individual participant data (IPD) sharing plan.
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| ID | Term |
|---|---|
| D006931 | Hyperbaric Oxygenation |
| ID | Term |
|---|---|
| D010102 | Oxygen Inhalation Therapy |
| D012138 | Respiratory Therapy |
| D013812 | Therapeutics |
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Erythrocytes found in peripheral blood.
| Two days, two different condition measurements |
| Hemoglobin (HGB) | Mean concentration of hemoglobin in the blood. | Two days, two different condition measurements |
| Hematocrit (HCT) | The percentage of red blood cells in the blood. | Two days, two different condition measurements |
| Mean corpuscular volume (MCV) | Mean volume of erythrocytes in the blood. | Two days, two different condition measurements |
| Mean corpuscular hemoglobin (MCH) | Mean mass of hemoglobin in an erythrocyte. | Two days, two different condition measurements |
| Mean corpuscular hemoglobin concentration (MCHC) | MCH expressed in a unit of blood volume. | Two days, two different condition measurements |
| Red cell distribution width (RDW) | Differences in the volume and size of erythrocytes. | Two days, two different condition measurements |
| Platelets (PLT) | Thrombocytes found in peripheral blood. | Two days, two different condition measurements |
| Plateletcrit (PCT) | Volume occupied by platelets in the blood. | Two days, two different condition measurements |
| Mean platelet volume (MPV) | Mean volume of thrombocytes in the blood. | Two days, two different condition measurements |
| Platelet distribution width (PDW) | Measurement of platelet anisocytosis calculated from the distribution of individual platelet volumes. | Two days, two different condition measurements |