The study aims to comparatively assess clinical parameters, biochemical and microbiological parameters after treatment of periodontitis using subgingival mechanotherapy traditional and subgingival mechanotherapy using a perioscope.

Non-surgical treatment is the basic form of disease treatment periodontitis. Its first stage is the mechanical removal of dental plaque along with the control of risk factors. Subgingival mechanotherapy, also known as scaling and root planing (SRP) is the next stage, aimed at the subgingival area. Perioscopy makes possible direct real-time visualization of the root surface during the procedure. The research results published so far indicate improvement or no significant differences clinical parameters after SRP procedure using a perioscope vs traditional subgingival instrumentation. The aim of this study is to indicate, or not, clinical, biochemical (level of pro-inflammatory cytokines) and microbiological (A. actinomycetemcomitans, P. gingivalis, T. denticola) differences after periodontitis treatment using the traditional method vs. using a perioscope.

Inclusion Criteria:

• age 20-60; (II)
• preserved lateral and anterior teeth;
• no periodontal treatment in the last 12 months;
• intermediate or advanced periodontitis (Stage II / III / IV)
• in each quadrant at least three teeth with PPD>5 mm after initial treatment (scaling, motivation, instruction)

Exclusion Criteria:

• antibiotic therapy in the last 6 months;
• smoking;
• systemic diseases affecting periodontal tissues;
• pregnancy or lactation;
• orthodontic treatment;
• tooth mobility II / III degree;
• involvement of third degree furcations according to Hamp classification;
• endodontic issues