Prospective multicenter longitudinal (observational) study recruiting from tertiary centers for the surgical management of esophageal cancer; Virginia Mason Medical Center (Seattle, USA) and St Mary's Hospital (Imperial College, London, UK). This is intended to be a pilot study.

Patients undergoing curative treatment for esophageal cancer will be recruited at the time of routine clinical assessment shortly following initial diagnosis and will undergo clinical and radiological evaluation at two study time points: (1) staging investigation (e.g. laparoscopy or endoscopy) and (2) definitive surgical resection. Study time points have been judiciously chosen to coincide with interventions that form part of patient's routine clinical care. It is intended that sampling will occur on the day of each intervention/surgery following a routine period of fasting.

Inclusion Criteria:

• Patients with the following characteristics will be eligible for inclusion in this study:

  • Age 18-90 years
  • Newly diagnosed (prior to treatment) with esophageal and/or gastroesophageal junctional cancer (adeno- or squamous cell carcinoma)
  • Planning to undergo curative treatment, including surgical resection with or without neoadjuvant therapy

Exclusion Criteria:

• Patients with the following characteristics will not be eligible for inclusion in this study:

  • Pregnant females
  • Without malignant esophageal disease
  • Inability or unwillingness to provided informed written consent

International cohort of patients presenting with regional {Stage 2 and 3} esophageal cancer who are considered appropriate candidates for surgical therapy.

Patient characteristics will be pulled out by three regions, North America, Europe and Asia. Additional anonymized data may be available upon request.