Microbe-host Interaction Relationship in Patients With Stroke-relatedPneumonia

Microbe-host Interaction Relationship in Patients With Stroke-related Pneumonia

This study intends to use a standardized cohort study to collect biological samples (including throat swabs, sputum and blood) from subjects eligible for inclusion, and use 16s DNA / metagenomic sequencing and metabolome technology to explore the microbial-host interaction relationship of SAP patients.

Stroke-related pneumonia (SAP) is the most common complication of acute cerebral hemorrhage, and it is one of the main causes of disease aggravation, prolonged hospitalization and costs, and even death. The traditional antibiotic model proved ineffective, and new prevention and treatment methods were urgently sought.This study intends to use a standardized and rigorous cohort study to collect biological samples (including throat swabs, sputum and blood) of eligible subjects, and to explore the microbial-host interaction relationship in patients with SAP, which may provide new targets and new ideas for the prevention and control of SAP.

Inclusion Criteria:

• Age: 18 years old;
• Stroke was confirmed by imaging;
• Within 48 hours of onset, the onset time cannot be determined from the last normal observation;
• Glasgow Coma Scale (GCS) 7-14;
• SAP by clinical symptoms, laboratory examination or imaging confirmation, the modified CDC diagnostic criteria;
• The patient (or his / her legal representative) signs a written informed consent form.

Exclusion Criteria:

• Use of antibiotics within 4 weeks before stroke onset;
• The presence of other conditions that may interfere with the follow-up and outcome assessment (such as known significant prestroke disability, mRS score of 3-5, end-stage malignancy, and liver and renal failure);
• Pregnant or lactating women.