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| Name | Class |
|---|---|
| Tropical Biome and ImmunoPhysiopathology (TBIP) - French Guiana University (Université de Guyane) | UNKNOWN |
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Intensive Care Unit (ICU) patients are exposed to catheter-related infections with an important morbidity. Catheter colonization is constant but infection is not. Cutaneous dysbiosis could be the missing link. Our study aims to evaluate the evolution of cutaneous microbiota in ICU patients with a central venous catheter in place, through metagenomics. Our main objective is to evaluate the evolution of alpha-diversity, quantified by intra-patient variation of Shannon diversity index (a diversity index used in bacterial metagenomics).
Central venous catheters (CVCs) are necessary in up to 60% of ICU patients, representing a risk of catheter-related infections with high morbidity and mortality. Catheter colonization originating mostly from the skin is constant, but infection is not. Dysbiosis is known to be associated with pathological states and infection, for example post-antibiotic C. difficile diarrheas, or atopic dermatitis, in which flares are associated with dysbiosis and S. aureus predominance. Cutanous dysbiosis could be the missing link between catheter colonization and infection. Our hypothesis is that under the influence of multiple ICU factors (stress, antibiotic administration, local dysinfection procedures), cutaneous dybiosis appears in ICU patients with a central venous catheter.
All adult ICU patients with an indication for CVC placement will be included over a 6 months period. Skin swabbing will be performed on CVC insertion site before CVC placement (baseline), and then every 3 days (or when dressing is changed) while CVC is in place, then at ICU discharge. Bacterial metagenomics using bacterial DNA extraction, 16S PCR amplification and Nanopore sequencing will allow for description of cutaneous microbiota and diversity evaluation through Shannon index. Evolution of alpha-diversity will be evaluated through time-series data analysis: comparison of Shannon index at various time points with baseline Shanonn index (before CVC placement). Standard microbiologic culture of skin swabbing will be performed. General patient characteristics and informations relative to CVC infection and treatment will be collected.
This study will have no impact on patient management.
Category 3 Non-Interventional Human Person Research (RIPH 3)
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Skin swabbing | Diagnostic Test | Skin swabbing will be performed on CVC insertion site before CVC placement (baseline), and then every 3 days (or when dressing is changed) while CVC is in place, then at ICU discharge. Bacterial metagenomics using bacterial DNA extraction, 16S PCR amplification and Nanopore sequencing will allow for description of cutaneous microbiota and diversity evaluation through Shannon index. Evolution of alpha-diversity will be evaluated through time-series data analysis: comparison of Shannon index at various time points with baseline Shanonn index (before CVC placement). Standard microbiologic culture of skin swabbing will be performed. | ||
| Data collection | Other | General patient characteristics and informations relative to CVC infection and treatment will be collected. |
| Measure | Description | Time Frame |
|---|---|---|
| Evolution of alpha-diversity | Comparison of Shannon index at various time points with baseline Shannon index (before Central Venous Catheter placement through leaving intensive care service). The higher the Shannon index, the greater the diversity. | Baseline |
| Evolution of alpha-diversity | Comparison of Shannon index at various time points with baseline Shannon index (before Central Venous Catheter placement through leaving intensive care service). The higher the Shannon index, the greater the diversity. | Day 3 |
| Measure | Description | Time Frame |
|---|---|---|
| Skin swabs Metagenomics | Metagenomics: Alpha and beta-diversity of skin swabs | Baseline |
| Skin colonization | Skin colonization: positive standard culture of skin swabs |
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Inclusion Criteria:
- Adult hospitalized in ICU at the Cayenne Hospital Center in whom the installation of a CVC is indicated
Exclusion Criteria:
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Adult hospitalized in ICU at the Cayenne Hospital Center in whom the installation of a CVC is indicated
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ariane ROUJANSKY | Contact | +594594395354 | ariane.roujansky@ch-cayenne.fr | |
| Hatem KALLEL | Contact | +594594395354 | hatem.kallel@ch-cayenne.fr |
| Name | Affiliation | Role |
|---|---|---|
| Ariane ROUJANSKY | Centre Hospitalier de Cayenne | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Centre Hospitalier de Cayenne | Recruiting | Cayenne | Guyane Française | 97306 | French Guiana |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 19246122 | Background | Tacconelli E, Smith G, Hieke K, Lafuma A, Bastide P. Epidemiology, medical outcomes and costs of catheter-related bloodstream infections in intensive care units of four European countries: literature- and registry-based estimates. J Hosp Infect. 2009 Jun;72(2):97-103. doi: 10.1016/j.jhin.2008.12.012. Epub 2009 Feb 25. | |
| 10395140 |
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| ID | Term |
|---|---|
| D064806 | Dysbiosis |
| D055499 | Catheter-Related Infections |
| D003428 | Cross Infection |
| ID | Term |
|---|---|
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D007239 | Infections |
| D007049 | Iatrogenic Disease |
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| ID | Term |
|---|---|
| D003625 | Data Collection |
| ID | Term |
|---|---|
| D004812 | Epidemiologic Methods |
| D008919 | Investigative Techniques |
| D017531 | Health Care Evaluation Mechanisms |
| D011787 | Quality of Health Care |
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Skin swab catheter (standard culture)
| Baseline |
| Catheter-related colonization | Catheter-related colonization: standard central line culture positivity | Baseline |
| Central Venous Catheter's Metagenomics | Metagenomics: alpha and beta-diversity of Central Venous Catheter at removal | Baseline |
| Catheter related Infection | Clinical signs and blood culture and/or positive catheter culture | Baseline |
| Skin swabs Metagenomics | Comparison of Shannon index at various time points with baseline Shannon index (before Central Venous Catheter placement through leaving intensive care service). The higher the Shannon index, the greater the diversity. | Day 3 |
| Skin colonization | Skin colonization: positive standard culture of skin swabs | Day 3 |
| Catheter-related colonization | Catheter-related colonization: standard central line culture positivity | Day 3 |
| Central Venous Catheter's Metagenomics | Metagenomics: alpha and beta-diversity of Central Venous Catheter at removal | Day 3 |
| Catheter related Infection | Clinical signs and blood culture and/or positive catheter culture | Day 3 |
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| D020969 | Disease Attributes |
| D017530 | Health Care Quality, Access, and Evaluation |
| D011634 | Public Health |
| D004778 | Environment and Public Health |