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The minimally processed diets of our ancestors have been rapidly replaced by UPFs driving poor diet to become the leading risk factor for preventable death globally. Hence, it is essential to understand what properties of UPF are driving their overconsumption to reduce diet-related mortality. To address this gap in knowledge this proposal will test:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Stimulus: UPF1 | Experimental | In a whole room metabolic chamber participants will consume a ~300 kcal meal consisting of ultra-processed foods as determined by the widely used NOVA scale. The whole room metabolic chamber will allow for collection of simultaneous measurement of multiple metabolic responses (glucose, insulin, and metabolic rate). Measures will be collected for 45 min before consumption of the meal (baseline) and for 3 hours after consumption (post-prandial). |
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| Stimulus: MPF1 | Active Comparator | In a whole room metabolic chamber participants will consume a ~300 kcal meal consisting of minimally processed foods as determined by the widely used NOVA scale. The whole room metabolic chamber will allow for collection of simultaneous measurement of multiple metabolic responses (glucose, insulin, and metabolic rate). Measures will be collected for 45 min before consumption of the meal (baseline) and for 3 hours after consumption (post-prandial). |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Ultra-processed food - Picture Set Meal | Other | A meal containing ultra-processed foods derived from the picture set used in the Becker-Degroot-Marschak auction paradigm. |
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| Measure | Description | Time Frame |
|---|---|---|
| Blood glucose response to level of food processing | Blood glucose concentration will be assessed at baseline and at set time points for 3 hours after consumption of each condition on different days. | Immediately post intervention |
| Blood insulin response to level of food processing | Blood insulin concentration will be assessed at baseline and at set time points for 3 hours after consumption of each condition on different days. | Immediately post intervention |
| Energy expenditure in response to level of food processing | Whole room indirect calorimetry will be used to determine energy expenditure at baseline and for 3 hours after consumption of each condition on different days. | Immediately post intervention |
| Respiratory exchange ratio in response to level of food processing | Whole room indirect calorimetry will be used to determine respiratory exchange ratio at baseline and for 3 hours after consumption of each condition on different days. | Immediately post intervention |
| Substrate oxidation in response to level of food processing | Whole room indirect calorimetry will be used to determine substrate oxidation at baseline and for 3 hours after consumption of each condition on different days. | Immediately post intervention |
| Effect of food processing on food value and encoding | A food picture set of 28 that are matched on 26 visual, perceptual, and nutritional properties but vary on degree of NOVA food processing level will be used in a Becker-Degroot-Marschak auction paradigm to assess participants' brain reward response for each food. |
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Inclusion Criteria:
Exclusion Criteria:
Claustrophobia (this would make lying in an MRI scanner or indirect calorimetry canopy very uncomfortable).
2. History of head injury resulting in loss of consciousness for more than 10 minutes 3. Current or past diagnosis of diabetes or metabolic disorder (thyroid disease, etc.) 4. Contraindications to MRI: Individuals with pacemaker, aneurysm clips, neurostimulators, cochlear implants, metal in eyes, steel worker, or other implants.
5. History of alcohol or drug dependence 6. Active neurologic disorder 8. Diagnosed eating disorder 9. Food allergies or restrictive diet
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Fralin Biomedical Research Institute at VTC | Roanoke | Virginia | 24016 | United States |
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| ID | Term |
|---|---|
| D008659 | Metabolic Diseases |
| ID | Term |
|---|---|
| D009750 | Nutritional and Metabolic Diseases |
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| Minimally Processed Food - Picture Set Meal | Other | a meal containing minimally processed foods derived from the picture set used in the Becker-Degroot-Marschak auction paradigm. |
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| through study completion, an average of 3 weeks |