Transplantation of Bone Marrow Mesenchymal Stromal Cells for Periodontal Regeneration

Transplantation of Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration in Periodontal Disease

The goal of this study is to evaluate the capacity of allogeneic bone marrow mesenchymal stromal cells (MSC) to induce bone regeneration in patients with periodontal disease. MSC cultured are loaded on a collagen scaffold, included into autologous platelet rich plasma clot and implanted in the bone defect.

The clinical protocol is designed to evaluate the bone regeneration capacity of allogeneic MSCs for the treatment of patients with chronic or aggressive periodontal disease. Patients with either a 2- or 3-wall intrabony defects are included in this study. A bioengineering construct, constituted by allogeneic MSCs and a collagen scaffold, is incorporated into platelet rich plasma (PRP) clot, which is implanted at the bone defect site. Follow up of treated tooth are assessed by clinical evaluation, intraoral radiography and cone-beam CT.

Inclusion Criteria:

• Medical healthy
• Minimum age of 18 years old
• Diagnosis of chronic or aggressive periodontitis
• Vertical intrabony defects of three or two walls
• PPD >= 5 mm
• Radiographic evidence of alveolar bone loss of at least 3 mm
• Minimim 20 teeth presents in mouth.
• Informed consent of the patient

Exclusion Criteria:

• Smoking
• Pregnancy or lactating
• Receiving immunosuppressive drugs, anticoagulants, antibiotics or analgesic drugs
• Diabetics