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The goal of this clinical trial is to compare the effects of protein consumption before sleep or immediately after exercise during 12 weeks of resistance exercise training in older adults. The main questions it aims to answer are:
Are there differences in muscle growth or strength during 12 weeks of resistance training when 40 grams of protein is consumed either before sleep or immediately after exercise? Are there differences in cognitive performance during 12 weeks of resistance training when 40 grams of protein is consumed either before sleep or immediately after exercise?
This study is a randomized double-blind placebo-controlled study. Participants will be randomly allocated into 1 of 3 groups. All three groups will perform the same 12-week resistance exercise training program two times per week and consume 40 g of protein: 1) Immediately post-exercise, 2) 30 minutes prior to sleep, or 3) no supplemental protein (control). Test of muscle growth and strength, and cognitive performance will be measure before, during, and after the exercise training program.
Researchers will compare the 3 conditions to determine if consume protein after exercise or before sleep during 12 weeks of resistance exercise training affects muscle growth and strength, and cognitive performance.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Post-exercise protein | Experimental | Consumed 40 g of supplemental protein immediately after exercise sessions and in between breakfast and lunch on non-exercise days. |
|
| Pre-sleep protein | Experimental | Consumed 40 g of supplemental protein 30 minutes before sleep every day. |
|
| Resistance exercise training only | Placebo Comparator | Did not consume supplemental protein. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Protein | Dietary Supplement | Post-exercise protein group consumed protein immediately after exercise sessions. Pre-sleep protein group consumed protein 30 minutes before sleep. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Muscle thickness | Change in muscle thickness of the quadriceps muscles in centimeters from week 0 (baseline) to week 6 (midpoint) to week 12 (end of study). | Week 0, 6, and 12 |
| Measure | Description | Time Frame |
|---|---|---|
| Maximum muscular strength | Changes in strength of the chest press and leg press exercises in kilograms from week 0 (baseline) to week 6 (midpoint) to week 12 (end of study). | Week 0, 6, and 12 |
| Muscle power |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Alex O Klemp, PhD | Student | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Florida State University | Tallahassee | Florida | 32306 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 40539259 | Derived | Klemp AO, Ormsbee MJ, Yeh M, Sokolowski CM, Kim DH, Panton LB, Kim JS. Neither pre-sleep nor post-exercise protein consumption influences resistance exercise training adaptations in older adults. J Int Soc Sports Nutr. 2025 Dec;22(1):2519511. doi: 10.1080/15502783.2025.2519511. Epub 2025 Jun 20. |
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| Type | Includes Protocol | Includes SAP | Includes ICF | Document Label | Document Date | Document Uploaded Date | Document File Name |
|---|---|---|---|---|---|---|---|
| Prot_SAP_ICF | Yes | Yes | Yes | Study Protocol, Statistical Analysis Plan, and Informed Consent Form | Jul 1, 2019 | May 27, 2023 | Prot_SAP_ICF_000.pdf |
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| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D006984 | Hypertrophy |
| ID | Term |
|---|---|
| D001519 | Behavior |
| D020763 | Pathological Conditions, Anatomical |
| D013568 | Pathological Conditions, Signs and Symptoms |
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| ID | Term |
|---|---|
| D011506 | Proteins |
| ID | Term |
|---|---|
| D000602 | Amino Acids, Peptides, and Proteins |
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| Placebo | Other | Resistance exercise training only group did not consume supplemental protein. |
|
Changes in power (Watts) during a sit-to-stand task from week 0 (baseline) to week 6 (midpoint) to week 12 (end of study).
| Week 0, 6, and 12 |
| Cognitive performance | Changes in the number of correct responses during the digit symbol substitution task and pattern comparison tests from week 0 (baseline) to week 12 (end of study). | Week 0 and 12 |
| Measures associated appetite | Changes in subjective measures of hunger, satiety, and desire to eat on a 0-100 millimeter (higher values indicating stronger feeling) visual analog scale from week 0 (baseline) to week 6 (midpoint) to week 12 (end of study). | Week 0, 6, and 12 |
| Total sleep time | Changes in actigraphy watch measured total sleep time in hours from week 0 (baseline) to week 12 (end of study). | Week 0 and 12 |
| Sleep latency | Changes in actigraphy watch measured sleep latency in minutes from week 0 (baseline) to week 12 (end of study). | Week 0 and 12 |
| Measures associated with sleep | Changes in actigraphy watch measured sleep efficiency (ratio of time spent asleep to the time spent in bed) in percentage from week 0 (baseline) to week 12 (end of study). | Week 0 and 12 |
| Nitrogen balance | Changes in urinary nitrogen balance (grams per day) from week 0 (baseline) to week 12 (end of study). | Week 0 and 12 |
| Free insulin-like growth factor 1 | Changes in plasma free insulin-like growth factor 1 concentration (nanogram per deciliter) from week 0 (baseline) to week 12 (end of study). | Week 0 and 12 |
| Mood | Changes in the Profile of Mood State rating scale categories of tension, depression, anger, fatigue, confusion, vigor, and total mood disturbance from week 0 (baseline) to week 12 (end of study) via a 1 to 5 scale (higher score indicates stronger feeling). | Week 0 and 12 |