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| Name | Class |
|---|---|
| Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca | OTHER |
| Hospital Universitario Virgen de la Arrixaca | OTHER |
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MNPLs effects on human health are still preliminary. Workers exposed to plastic residues are exposed constantly to MNPLs. One of these jobs corresponds to greenhouse workers, that could be considered as a population highly exposed to plastics. The objective of the research is to be able to detect MNPLs on body fluids as well as their potential genotoxic and immunological damage.
The exponential increase in the production/use of plastic translates into a parallel increase of environmental plastic waste that is continuously degraded into MNPLs. Information on the MNPLs' effects on human health is still preliminary and, furthermore, the limitations in current methodologies prevent accurate exposure/risk assessment.
This observational study will obtain body samples of greenhouse workers and from a control group. That would allow optimizing specific biomarkers of human MNPLs exposure and the characterization of biomarkers of early effect that allows an association among the exposure to MNPLs and effects on human health.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control | Biomonitoring studies to detect MNPLs in biological samples and study of health effects. Determination of MNPLs levels, genotoxic damage, and immunological effects |
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| Greenhouse workers | Biomonitoring studies to detect MNPLs in biological samples and study of health effects. Determination of MNPLs levels, genotoxic damage, and immunological effects |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Biomonitoring of MNPLs in biological samples of greenhouse workers and controls | Other | Detection of biomarkers of exposure and genotoxic effect in biological blood, urine, exhalated air condensate, and feces |
| Measure | Description | Time Frame |
|---|---|---|
| Biomonitoring study to detect the presence of MNPLs in body fluids | Blood, buccal cells, exhalate breath condensate, feaces and urine will be collected from donors to check for the presence of micro and nanoplastics and/or derivatives | Up to 1 year |
| Genotoxicity effects of exposure to MNPLs | Blood and buccal cells will be collected from donors to check for the presence genotoxic permanent damage, assessed by the micronucleus assay | Up to 1 year |
| Genotoxicity effects of exposure to MNPLs | Blood will be collected from donors to check for the presence genotoxic non-permanent damage, assessed by the comet assay | up to 1 year |
| Study of the effects of MNPLs on immune system | Blood will be collected from donors to check if the presence of micro and nanoplastics have an effect on th function of the immune system (signalling and activation) in vivo and in high-risk individuals | Up to 1 year |
| Measure | Description | Time Frame |
|---|---|---|
| Surrogate biomarkers after MNPLs exposure | If it is possible to correlate the presence of micro and nanoplastics with the presence of plastic derivatives in body fluids, as blood or urine, this will facilitate the detection of plastic, because tecniques to detect surrogate biomarkers as e.g. plasticizers or antioxidants are cheaper and most use than the tecnology to detect MNPLs. For that different analytical tecniques will be used. |
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Inclusion Criteria of workers exposed to plastic residues:
Inclusion Criteria of controls :
Exclusion Criteria of workers exposed to plastic residues:
Exclusion Criteria of controls:
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The investigators will study adult professionals working in greenhouses in the countryside of Cartagena and Almeria. At least 25 volunteer professionals willing to participate in the study will be evaluated.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ricard Marcos | Contact | +34 93 586 20 52 | ricard.marcos@uab.cat |
| Name | Affiliation | Role |
|---|---|---|
| Pablo Pelegrín | Instituto Murciano de Investigación Biosanitaria (IMIB) | Principal Investigator |
| Juan Bernardo Cabezuelo | Hospital Clínico Universitario Virgen de Arrixaca (Murcia) | Study Chair |
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| ID | Term |
|---|---|
| D007249 | Inflammation |
| ID | Term |
|---|---|
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
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Peripheral blood, urine, feces
| Up to 1 year |
| Laura Martínez | Hospital Clínico Universitario Virgen de Arrixaca (Murcia) | Study Chair |