Periodontal regenerative therapy has focused on various non-surgical and surgical treatment methods. Platelet-rich fibrin (PRF) has shown to possess multiple uses, including properties that promote healing of both soft and hard tissues. In the present study, we hypothesized that the addition of PRF into periodontal pockets, as an adjunct to conventional scaling and root planing (SRP), would enhance the outcomes of non-surgical periodontal therapy. This split-mouth randomized controlled clinical trial included 26 periodontal pocket sites in 13 patients with periodontitis. The pockets were randomly assigned as either test or control sites. In the test group, PRF was placed as an adjunct to SRP, while the control group received SRP alone. Probing pocket depths (PPD), clinical attachment levels (CAL), gingival recession (GR), plaque index (PI), and gingivitis index (GI) were measured at baseline and at six weeks. The wound healing index was measured during the six-week follow-up period.

Inclusion Criteria:

• either gender,
• aged 18 years or older,
• patients without a medical history of systemic diseases,
• patient with chronic periodontitis
• patient with 5 or 6 mm of probing depth

Exclusion Criteria:

• patients with medical history of systemic disease or bleeding disorders
• presence of other gingival diseases (such as leukoplakia, lichen planus, pemphigoid disorders, pemphigus vulgaris, herpetic lesions, Necrotizing Ulcerative Periodontitis (NUP)
• pregnancy
• history of any drug usage affecting the periodontium for the past six months (such as systemic antibiotic therapy)
• previous periodontal treatment in the last six months
• smoking
• teeth with untreated caries
• endodontic lesions and grade II or more mobility
• subjects with acute exacerbation of periodontitis
• patients with systemic
• disease or condition that could affect tissue healing (e.g., autoimmune disease)
• severe furcation involvement (grade II and III)
• abutment for prosthetic rehabilitation
• active orthodontic therapy

There will be not necessary