Cell Free DNA Quantification in Patients With Endometriosis Followed Witd Medical Assistance to Procreation

Cell Free DNA Quantification in Patients With Endometriosis Followed Witd Medical Assistance to Procreation

Endometriosis is a common benign disease in premenopausal women and causes chronic pelvic pain and infertility. This infertility may be due to pelvic adhesions and surgery but also because of poor oocyte quality. It is known that endometriosis is associated with an increase oxidative stress, wich induce chronic inflammation, deleterious effect for DNA, proteins and can caused cellular death. ROS markers found in follicular fluid or in serum are significatively higher in endometriosis women. The investigators want to dose a marker of apoptosis in infertile women and see if it's significatively higher in serum and in follicular fluid of patients with endometriosis compared to others infertility causes and if it's correlated to oocyte quality and IVF results. Real time PCR will be used to dose cell free DNA in serum and follicular fluid of patients undergoing IVF treatment (endometriosis and infertility due to tubal factor, male infertility or idiopathic cause). Then the investigators will compare cell free DNA rate with oocyte and embryo quality and with pregnancy outcomes in the different group.

Inclusion Criteria:

• patients eligible medical assistance to procreation who are about to undergo IVF treatment
• aged between 18 and 43 years old
• and who have signed a consent form

Exclusion Criteria:

• patients who are not eligible for medical assistance to procreation,
• patients who are followed for other inflammatory disease,
• patients followed for premature ovarian failure or
• patients with a recent story of pelvic infection