This study evaluates the levels of biomarkers associated with bone metabolism in patients with different periodontal conditions.

Background: In this study, the levels of bone metabolism markers found in the saliva of individuals with different periodontal conditions were compared and the relationship of these markers with periodontal status was examined.

Method: A total of 60 individuals, 20 with periodontitis (P), 20 with gingivitis (G), and 20 with periodontal healthy (S), were included in the study. Periodontal clinical parameters (plaque index, gingival index, bleeding on probing, clinical attachment level and probing pocket depth) and saliva samples were obtained from the individuals. Bone metabolism biomarker (necrotizing factor (TNF)-α, osteoprotegerin (OPG), nuclear factor kappa-β receptor activator ligand (RANKL) and sclerostin) levels in saliva samples were evaluated by enzyme-linked immunosorbent assay (ELISA).

Inclusion Criteria:

• 18-65 years old.
• not having any systemic disease that may affect periodontal status
• not smoking and alcohol use
• volunteer to participate in the study

Exclusion Criteria:

• periapical pathologies;
• resulting from orthodontics and occlusion extreme forces, including mechanical forces;
• chronic high-dose steroid therapy;
• radiation or immunosuppressive therapy;
• pregnancy;
• breastfeeding;
• allergy or sensitivity to any medication.
• Periodontal therapy or drug therapy for at least 6 months of study participants