Microscopy Imaging of Whole-mount Stained Human Tissues

Microscopy Imaging of Whole-mount Stained Human Tissues: Development of an Imaging Platform for Intraoperative Tumor Margin Delineation

The goal of this clinical trial is to develop an imaging platform for intraoperative tumor margin delineation in 250 cases of tumor-suffered patients. The main questions it aims to answer are:

• to develop the protocol of rapid assessment of surgical specimens without need for fixation, embedding, and cryosectioning required for conventional histopathology.

Participants will provide a small piece of their surgical specimens from tumor removal surgery .

If there is a comparison group: Researchers will compare normal specimens to see if we can observe the difference.

Inclusion Criteria:

1. Patients who are more than 20 years old.
2. Diagnosed as patients suffered by skin tumor, brain tumor and breast tumor.

Exclusion Criteria:

1. Patients who can't speak and communicate with others.
2. Patients who can't make eventual and exact pathological assessment for his/her diseases.
3. Patients who only can fit for slicing tumor.
4. Removed tissues which obviously vary after severe ray radiation under microscopy observation.
5. Diameter of tumors are under 1 centimeter.
6. Breast carcinoma in situ.