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The purpose of this study will be to assess the effectiveness of bioimpedance device (BIA) as a safe, rapid, and non-invasive method of analyzing muscle damage induced by high intensity induced eccentric exercise as an alternative measurement tool against traditional assessment methods.
A sample of 28 sedentary subjects will be recruited, all participants will perform an eccentric exercise protocol.
Measurements will be done before performing the exercise protocol, immediately after finishing the protocol, 3h after the end of the exercise protocol, 24h after the end of the exercise protocol and 48h after the end of the exercise protocol.
The variables analyzed in all subjects will be phase angle, creatinkinase, jumping strength, VAS scale, white blood count cell, quadriceps muscle thickness, Borg scale and anxiety and depression questionnaires.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Single group: excentric exercise protocol | Experimental | Excentric exercise protocol will be performed for all subjets, consisted of 10 sets of 20 repetitions of squats, jumping from a height of 50 cm |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Experimental: Single group: excentric exercise protocol | Other | Excentric exercise protocol will be performed for all subjets, consisted of 10 sets of 20 repetitions of squats, jumping from a height of 50 cm |
| Measure | Description | Time Frame |
|---|---|---|
| Phase angle | The phase angle, which is measured via bioelectrical impedance analysis (BIA), InBody 770 ,is a clinically important bioimpedance parameter used for nutritional assessment and evaluating the risk of various diseases. The phase angle was calculated automatically by the BIA device from these two components according to the following formula: phase angle (°) = (reactance/resistance) × (180°/π). | change from baseline to 72 hours |
| Measure | Description | Time Frame |
|---|---|---|
| Pain: VAS | Visual Analogic Scale. Pain will be measured with a visual analog scale from 0 to 10. 0 points is no pain. | change from baseline to 72 hours |
| Quadriceps thickness | Quadriceps muscle thickness will be measures in mm. by an ultrasound device |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Jorge Buffet García | Contact | +34619903972 | j.buffet.prof@ufv.es |
| Name | Affiliation | Role |
|---|---|---|
| Jorge Buffet García | Universidad Francisco de Vitoria | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Universidad Francisco de Vitoria | Recruiting | Pozuelo de Alarcón | Madrid | 28223 | Spain |
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| change from baseline to 72 hours |
| Creatinkinase (CK concentration) | CK concentration will be measure in mg/dl with a portal analyzer (SpotchemTM EZ SP-4430), using test strips. | change from baseline to 72 hours |
| cellular inflammatory markers | Cellular inflammatory markers will be measured with hematology analyzer (Sysmex KX-21N Automed) in 10^9/L and % White blood count, lymphocytes, neutrophils, monocytes, eosinophils, basophils will be analyzed | change from baseline to 72 hours |
| Leg power | Leg power will be assessed using vertical jump test, with Chronojump platform (CHRONOJUMP Boscosystem) | change from baseline to 72 hours |