In order to avoid under- and over-arousal, participants will have the opportunity to choose between several documentaries. The participants will also receive a list of the available documentaries (based on selections from Netflix and Disney+) after the familiarization trial so that the trial can go as smoothly as possible.

Reaction time on the stroop will be assessed to determine behavioural induction of mental fatigue.

Accuracy on the stroop will be assessed to determine behavioural induction of mental fatigue.

Electroencephalography (EEG) will be used to continuously measure brain activity by using 32 active Ag/AgCl electrodes attached on the participants' head (Acticap, Brain Products Munich, Germany), according to the "10:20 International System". The sampling rate will be set at 500 Hz (Brain Vision Recorder, Brain Products, Munich, Germany). Electrode impedance will be kept <10 kΩ throughout the recording. Baseline measurements will be taken 2 min with eyes open, 2 min with eyes closed and subjects will be seated in a sound isolated dimly lit room. Afterwards, Event-related potentials (ERP), spectral power and brain connectivity analysis will be executed. The aim of the EEG device is to measure the presence of MF during the Stroop task, and to identify possible mechanisms behind the suspected decreases in human performance.

Age will be assessed to determine influences on mental fatigue effects

Length will be assessed to determine influences on mental fatigue effects (using SECA 206, SECA Benelux, The Netherlands)

Weight will be assessed to determine influences on mental fatigue effects (using Tanita TBF 300, Tanita Europe BV, The Netherlands)

Sex will be assessed to determine influences on mental fatigue effects

Fat percentage will be assessed to determine influences on mental fatigue effects (Tanita TBF 300, Tanita Europe BV, The Netherlands)

General health will be assessed using the Burn out assessment tool (BAT). The questionnaire assesses 4 core domains in burn out: exhaustion, emotional impairment, cognitive impairment and mental distance Moreover, 2 additional domains that assess specific symptoms of burn out are also assessed: psychosomatic complaints and psychological distress.

Additionally, a questionnaire that determines recovery need will be added to the burn out assessment. For this part, we will use the recovery need section of the "Short Inventory to Monitor Psychosocial Hazards" questionnaire.

Objectively, a graded maximal cycle ergometer test will be performed in 18-20°C and a RH of 40%, to attain the maximal exercise ability. This ability will then be translated to a specific performance level as defined by De Pauw et al.

Subjectively, the international physical activity questionnaire short form (IPAQ-SF) will assess the overall physical activity habits of the participant. The IPAQ-SF consists of 7 questions, assessing amount and level of intensity of physical activity in the last 7 days, and represents good reliability and validity.

a specific questionnaire will be designed with questions examining sport participation. These answers on these questions will then be employed to divide participants into a tier based system as proposed by McKay et al.

Working memory will be assessed using a short n-back task. Participants will be instructed to focus on a white computer screen with a randomly generated number between 0-9. They have to press a button if the number in front of them matches the number they encountered 2 numbers earlier (2 back paradigm). Stimuli are presented for 500 ms, with an interstimulus time of 2500 ms, and a target/non target ratio of 33/67 (58). Outcomes include ACC (percentage of correct responses).

To examine response inhibition, participants will be required to perform a sustained attention to response task (SART). Participants were presented with single digits in the centre of the screen, ranging from 0-9, and were instructed to react as fast as possible if a number was shown on screen. However, if the number that was displayed equalled 3, participants were asked to refrain from responding. To specifically measure response inhibition, the outcome of this task consisted of the percentage of correct responses to the number 3 stimulus.

Attention will be assessed using a psychomotor vigilance task (PVT). The task features a red triangle in the centre of a computer screen, where participants are required to react as fast as possible when a yellow count is started in the middle of this red triangle. The interstimulus time ranges between 2 and 10 sec. Mean RT is collected as outcome, and false alarms (>200 ms reaction) and misses (>900 ms) are also measured.

Genes will be investigated using blood samples which will be captured in EDTA tubes and stored at -80 °C at the lab. The blood samples will be taken in between the physical assessment and the maximal incremental exercise test. One sample will then be sent to BRIGHTcore where the DNA will be extracted and analysed using exome sequencing.

Sleep quality will be assessed using the Pittsburgh Sleep Quality Index (PSQI). The PSQI's 19 self-reported items belong to one of seven subcategories: subjective sleep quality, sleep latency, sleep duration, habitual sleep efficiency, sleep disturbances, use of sleeping medication, and daytime dysfunction. The initial evaluation of the index found a reliability of 0.83 (good), and a test-retest reliability of 0.85 (good). Overall, the PSQI shows excellent reliability and validity for both clinical as well as non-clinical samples.

Sleep profile will be assessed using the single item chronotype scale (SIC). The SIC features six images relating to different chronotypes, and participants are required to select the picture that most closely relates to their perceived chronotype ("Which image best corresponds to your activation level during the day (i.e. in the morning, during daytime/afternoon, in the evening)?").

Trait self-control will be assessed using brief self-control scale developed by Tangney et al.. This 13 item scale is designed to assess trait self-control and has been shown to be correlated with the total self-control scale. It supports questions such as "I am good at resisting temptation" where participants are instructed to indicate if this statement is "not at all" (1) to "very much" (5) applicable to themselves.

The state-trait anxiety inventory involves two 20-item subscales describing trait and state anxiety, respectively. Only the first subscale will be used in this study. A score between 1 and 4 (from "Not at all "to "Very much so ") has to be provided on every item. The higher the score, the more severe the trait anxiety.

The eight item mental toughness questionnaire designed by Gucciardi et al. will be used asses MT. Participants will be asked to indicate how true each of the statements are (e.g., "I strive for continued success"), using a seven-point Likert scale (1-7) where 1 is designated as "false, 100% of the time" and 7 is designated as "true, 100% of the time".

Will be determined using the revised caffeine consumption questionnaire (CCQ-R). This questionnaire is designed to assess the approximate self-reported consumption of caffeine during the week, with products ranging from coffee and tea to food containing caffeine and drugs, and takes about 5 min to complete. The CCQ-R is a valid way to assess caffein consumption.

Motivation will be assessed using a VAS. Participants will be asked "How motivated do you feel for the upcoming task", and will be instructed to rate their answer on a 10cm line, with the statements "not at all" and "extremely motivated" at opposite ends of this line. Since motivation has often been cited as a potential mechanism behind MF, its measurement might give us a clue about the mechanisms behind possible influencing individual factors. Moreover, multiple MF studies have already used a VAS as a measuring method for motivation.

The Brunel Mood Scale (BRUMS) will be used to indicate mood, and serve as a control measure for both arousal as well as boredom . It will be assessed before and after each trial. This scale is based on the Profile of Mood States, and was validated using a multi-sample confirmatory factor analysis. It features 24 mood descriptors (divided into 6 subscales (i.e. anger, confusion, depression, fatigue, tension and vigor)), where participants have to give a value for every mood on a 5-point Likert scale (i.e. 0=not at all to 4 = extremely) responding to the question: "how do you feel right now?". It supports an adequate internal consistency and test-retest reliability. Moreover, it has been used in multiple MF studies and can accurately be used as a possible confounder variable.

a P-VAS scale (0-10 cm) will be administered before, during (every 3 min) and at the end of each time trial to check the level of subjective physical fatigue of the participants. Participants will be asked to rate their general physical fatigue on a 10 cm line ranging from "not at all" to "completely exhausted". The validity and reliability of a visual analogue scale to assess general fatigue was demonstrated by Lee et al.

The Karolinska Sleepiness Scale (KSS) will be conducted at the start of every trial, and before and after the intervention/control trial. This 10-point scale measures the subjective level of sleepiness and goes from 1 = 'extremely alert' to 10 = 'extremely sleepy, falls asleep all the time'. It is an often used scale in sleepiness research, Kaida et al. stated that this scale has a high validity in measuring sleepiness.

To assess the feeling of boredom the question "How boring did you find the performed task?" will be asked, the subjects will have to answer on a visual analogue scale that ranges from 'not at all' to 'completely bored'. This visual analogue scale for boredom (B-VAS) will be taken after each cognitive task, the intervention/control trial and the physical task. The validity and reliability of a general visual analogue scale has already been determined, and the B-VAS has also already been used in MF research.

the internal load of the physical performance measure will be examined using the CR100 RPE scale. This scale is a valid and modified version of the original Borg RPE measure, depicting a scale of 1-100 with verbal anchors reading "nothing at all", "minimum", "extremely weak", "very weak", "weak", "moderate", "strong", "very strong", "extremely strong" and "maximal". These verbal anchors are subsequently divided into larger categories (i.e. "just noticeable", "light", "heavy", "max"). Moreover, an "absolute maximum" is also depicted, which is displayed far above the so called maximum. The CR100 scale is a reliable and valid measure of internal load in physical performance, and is hypothesized to be more sensitive to changes in internal load compared to the CR10 scale.The RPE will be examined before, after and throughout (2 min intervals) the time trial.

Glucose concentration (mmol/l) (Bayer Contour Next USB Glucosemeter, Leverkusen, Germany) will be measured both before and after the cognitive- and physical outcome tasks. Subject's will be asked to give a 0.6-µl sample of whole blood from the left index finger top for assessment.

Heart Rate will be assessed by using the Equivital™ Life Monitor EQ-02 Physiological Status Monitor (PSM). Heart rate will be assessed during each run of the go/NoGo taks, every block of the Stroop task and the entirety of the time trial. Data will be averaged over each task and for every block of the Stroop task.

blood lactate will be continuously measured every 3 min during the performed time trial and at the beginning and end of the trial at the right ear lobe of the participant (determined enzymatically; EKF; BIOSEN 5030, Magdeburg, Germany). It will also always be measured at the beginning and end of the physical task.