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The incidence of age-related diseases, such as cancer and cardiovascular diseases, rapidly rises. These days, age-related diseases cause the majority of all healthcare expenses. During ageing senescent cells secrete a range of harmful inflammatory signals, which leads to chronic inflammation (inflamm-aging) and accelerates aging throughout the rest of the body. Interestingly, people who regularly exercise show less signs of inflamm-aging compared to people with a sedentary lifestyle. Molecules secreted by skeletal muscles, called myokines, may play an important role. However, the underlying molecular mechanisms remain unknown. This pilot study aims to investigate if an acute bout of resistance training is an appropriate tool to induce an increase in the release of muscle derived myokines in aged persons.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Aged individuals who perform exercise training | Active Comparator | Aged individuals will perform an acute bout of resistance trainig (30 minutes). This training is focused on the lower limbs. |
|
| Aged individuals who stay sedentary | Sham Comparator | Aged individuals will remain seated for 30 minutes. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Exercise training | Other | Resistance training of the lower limbs |
|
| Measure | Description | Time Frame |
|---|---|---|
| Cytokine concentration | Measurement of multiple cytokines in blood (for example: IL-6, IL-15, IL-4, irisin...) with ELISA's or multiplex assays | Day 2 |
| Myokine production | Immunohistochemistry staining (fluorescent) of multiple myokines (for example: IL-6, IL-15, IL-4, irisin..) and aging markers (p16,p21...) on muscle tissue | Day 2 |
| Immune parameters | Flow cytometry panels of immune parameters in muscle tissue | Day 2 |
| Myokine protein levels | Western blotting of multiple myokines in muscle tissue | Day 2 |
| Myokine gene expression | Quantitative PCR (polymerase chain reaction) of markers in muscle tissue | Day 2 |
| Measure | Description | Time Frame |
|---|---|---|
| Blood pressure | The force of circulating blood on the walls of the arteries. Measured using an electronic sphygmomanometer | Day 1 |
| Resting Heart Rate | Measured using an electronic sphygmomanometer |
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Inclusion Criteria:
Exclusion Criteria:
Smoking
Infection < 2 weeks ago
Received a vaccine < 2 weeks ago
Allergy to Lidocain
Immune deficiency
Presence of a tumour or in treatment
Usage of anti-coagulant medication
Presence of following conditions:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Bert Op'T Eijnde, Prof. dr. | Contact | +3211292121 | bert.opteijnde@uhasselt.be | |
| Chiel Hex, MD. | Contact | chiel.hex@uhasselt.be |
| Name | Affiliation | Role |
|---|---|---|
| Chiel Hex, MD. | University of Hasselt | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Hasselt University | Recruiting | Diepenbeek | Limburg | 3590 | Belgium |
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| ID | Term |
|---|---|
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D001519 | Behavior |
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| ID | Term |
|---|---|
| D015444 | Exercise |
| D011795 | Surveys and Questionnaires |
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
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Aged individuals who perform exercise training are compared to aged individuals who remain sedentary.
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| Questionnaire | Behavioral | Physical activity questionnaire |
|
| Percutaneous muscle biopsy | Procedure | Muscle biopsy of the vastus lateralis (m. quadriceps) |
|
| Blood sample | Procedure | Venous blood sample |
|
| Day 1 |
| International Physical Activity Questionnaire (Short form) | Measures the subjective level of activity of the last seven days. 7 questions regarding amount of time spent on certain activities. | Day 1 |
| D003625 | Data Collection |
| D004812 | Epidemiologic Methods |
| D008919 | Investigative Techniques |
| D017531 | Health Care Evaluation Mechanisms |
| D011787 | Quality of Health Care |
| D017530 | Health Care Quality, Access, and Evaluation |
| D011634 | Public Health |
| D004778 | Environment and Public Health |
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |