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| Name | Class |
|---|---|
| Ansh Labs | UNKNOWN |
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The research aims to characterize changes in ovarian and pituitary hormones associated with the development of ovarian follicular waves during the human menstrual cycle.
Our laboratory's previous research has demonstrated that multiple waves of ovarian follicles develop throughout the menstrual cycle. Approximately 2/3 of women develop 2 follicle waves throughout their cycle while the remainder exhibit 3 waves of follicle growth (1,2). This study is to follow up on those findings to determine how pituitary and ovarian hormones regulate the development of follicle waves in women, with a combination of ultrasound scans and blood and urine sampling to characterize how follicles grow during the menstrual cycle in 50 women of reproductive age. Changes in follicle growth across the cycle will be compared with changes in hormone production.
The new findings will be essential for improving understanding of female reproduction, with clinical applications in contraception, fertility and menopausal care for women across their reproductive lifespan.
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Transvaginal ultrasound scans | Diagnostic Test | Transvaginal ultrasound scans to map ovarian follicle growth and ovulation, finger-prick blood sampling for dried blood spot (DBS) hormonal assays and urine sampling for hormone metabolites, every consecutive day for an interovulatory interval. Weekly venipuncture samples will be taken for standard ELISA hormonal assays. The hormones of interest are FSH, LH, estradiol, progesterone, AMH, inhibins A and B, GDF-9 and BMP-15. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Antral follicle diameter | Daily diameters of individual follicles ≥ 2mm from ultrasound examination | One menstrual cycle (21-35 days) per volunteer |
| Antral follicle count | Daily count of the number of follicles >2 mm from ultrasound examination | One menstrual cycle (21-35 days) per volunteer |
| Follicle stimulating hormone (FSH) serum level | Daily measurements of FSH in IU/L | One menstrual cycle (21-35 days) per volunteer |
| Luteinizing hormone (LH) serum level | Daily measurements of LH in IU/L | One menstrual cycle (21-35 days) per volunteer |
| Estradiol serum level | Daily measurements of estradiol in pg/mL | One menstrual cycle (21-35 days) per volunteer |
| Progesterone serum level | Daily measurements of progesterone in ng/mL | One menstrual cycle (21-35 days) per volunteer |
| Anti-mullerian hormone (AMH) serum level | Daily measurements of AMH in ng/mL | One menstrual cycle (21-35 days) per volunteer |
| Inhibin A serum level | Daily measurements of inhibin A in pg/mL |
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Inclusion Criteria:
Exclusion Criteria:
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Volunteers who respond to advertisements placed in the community, local offices and the University of Saskatchewan's website
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| Name | Affiliation | Role |
|---|---|---|
| Angela R Baerwald, PhD,MD,CCFP | University of Saskatchewan | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Saskatchewan | Saskatoon | Saskatchewan | S7N 5A2 | Canada |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 12849812 | Background | Baerwald AR, Adams GP, Pierson RA. A new model for ovarian follicular development during the human menstrual cycle. Fertil Steril. 2003 Jul;80(1):116-22. doi: 10.1016/s0015-0282(03)00544-2. | |
| 12748128 | Background | Baerwald AR, Adams GP, Pierson RA. Characterization of ovarian follicular wave dynamics in women. Biol Reprod. 2003 Sep;69(3):1023-31. doi: 10.1095/biolreprod.103.017772. Epub 2003 May 14. |
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| ID | Term |
|---|---|
| D018962 | Phlebotomy |
| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
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Dried blood spots, serum frozen at -80 C and dried urine spot samples
| One menstrual cycle (21-35 days) per volunteer |
| Inhibin B serum level | Daily measurements of inhibin B in pg/mL | One menstrual cycle (21-35 days) per volunteer |
| D003933 | Diagnosis |
| D011677 | Punctures |
| D013812 | Therapeutics |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |