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| Name | Class |
|---|---|
| Valio Ltd | INDUSTRY |
| Chr Hansen | INDUSTRY |
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The aim of this study was to investigate the host-microbe interaction effects of various Lacticaseibacillus rhamnosus GG strains, with different binding capacities to the mucus in the small intestine, in healthy individuals
The project consisted in the supplementation of a) a wild-type LGG strain, b) a low-producing pili LGG strain or c) a high-producing pili LGG strain to study subjects. The individuals received the three treatment combinations in a randomised order. Each supplementation was given to the individuals every 30 min over a period of 6 hours. Eight biopsies from the duodenum were collected at the end of 6 h-solutions administration by flexible gastroduodenoscopy, likewise the routine procedure during this endoscopic investigation. Faecal samples were collected by the study subjects at home 1 day before, 2, 7 and 14 days days after administrations of each oral supplementation. The supplementations were separated by a 3-week wash-out period.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Wild-type | Active Comparator | Supplementation with the wild-type of the LGG strain |
|
| Low-pill | Experimental | Supplementation with the low-pili LGG strain |
|
| High-pili | Experimental | Supplementation with the high-pili LGG strain |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Probiotic | Dietary Supplement |
|
|
| Measure | Description | Time Frame |
|---|---|---|
| Difference in whole genome gene expression of duodenal mucosa after administration of three different LGG strain | Microarray-based Gene expression Analysis were carried out from duodenal biopsies collected after the supplementation of each LGG strain | 6hrs supplementation after start of LGG strain administration. |
| Microbiome of duodenal mucosal biopsies | Bacterial composition was analysed by Illumina MiSeq sequencing the hypervariable V3-V4 region of the 16S rRNA gene | 6hrs supplementation after start of LGG strain administration |
| Measure | Description | Time Frame |
|---|---|---|
| Faecal microbiome | Bacterial composition by MiSeq sequencing hypervariable V3-V4 region of 16SrRNA gene | 2 days after LGG strain administration |
| Faecal microbiome | Bacterial composition by MiSeq sequencing hypervariable V3-V4 region of 16SrRNA gene |
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Inclusion Criteria:
Exclusion Criteria:
Recent or current treatment with drugs affecting intestinal function, immune function or mood (e.g. antidepressants). In addition use of all drugs during the study should be recorded.
Recent (< 6 weeks) use of probiotics*.
Diagnosis of major psychiatric or somatic disorders.
Pregnancy or breastfeeding.
Abuse of alcohol or drugs.
Smokers and chewable tobacco users.
Lactose intolerance.
Dairy products:
Dietary supplements:
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| Name | Affiliation | Role |
|---|---|---|
| Robert Brummer, Professor | Örebro University, School of Medical Sciences, Sweden | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Örebro University | Örebro | 703 62 | Sweden |
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| ID | Term |
|---|---|
| D019936 | Probiotics |
| ID | Term |
|---|---|
| D019587 | Dietary Supplements |
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
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The individuals did receive the three treatment combinations in a randomised order.
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The sachets containing the different LGG strains were labelled as A, B, and C. The codes for each strain were kept by the industrial partner that provided the probiotic strains. Only at the end of the data analyses were the codes disclosed
| 7 days after LGG strain administration |
| Faecal microbiome | Bacterial composition by MiSeq sequencing hypervariable V3-V4 region of 16SrRNA gene | 14 days after LGG strain administration |
| D019602 |
| Food and Beverages |