Pregnancy Rate in Direct Versus Afterload Technique of Embryo Transfer

Pregnancy Rate in Single Step Versus After Load Technique of Embryo Transfer: a Retrospective Analysis

The primary outcome of the study is to determine if a difference in terms of pregnancy rate exists between direct and afterload embryo transfer (ET) techniques.

The secondary end points include the evaluation of the difficult transfer rates.

The study database includes all the single fresh and frozen (G5-G6) blastocyst transfers performed at Humanitas Fertility Centre between 1st January 2016 and 31th December 2021 by using the direct and afterload protocols.

Any blastocysts that underwent preimplantation genetic testing will be excluded from the analysis, as well as embryos derived from gamete donation or those imported from other centers.

Data are gathered using a specific internal web-based database, which allows to organize, store and readily retrieve information about patients and IVF cycle.

Inclusion Criteria:

• IVF procedure
• Fresh and frozen single blastocyst transfer

Exclusion Criteria:

• Any blastocysts that underwent PGS testing
• Embryos derived from gamete donation
• Embryos imported from other centers