Endocrine hormones, not only blood glucose responsive hormones, but also sexual hormones, thyroid hormones, growth hormones and so on, may have response and activites in hypoglycemia. However, the different responses of endocrine hormones to hypoglycemia in patients With diabetes remain unclear.

This study will observe the changes of insulin, C-peptide, glucagon, growth hormone, IGF-1, ACTH, cortisol, thyroid function, sexual hormone in newly diagnosed men with type 2 and type 1 diabetes mellitus using hyperinsulinaemic hypoglycaemic clamp technique

Inclusion Criteria:

- Patients

• newly diagnosed male patients with type 1 or type 2 diabetes (diagnostic criteria of World Health Organization)
• aged 18-50 years
• has not been treated with any hypoglycemic drugs
• HbA1c>9% Normal subjects: healthy and had no known illness

Exclusion Criteria:

• patients with insulin allergy.
• any history of hypoglycemic agent intake or were presently taking hypoglycemic agents
• any abnormality in liver or kidney function on blood and urine investigations
• any history of systemic corticosteroid use in 3 months
• any recent infections or acute medical events
• any other obvious conditions or associated diseases determined by the researcher: such as severe cardiopulmonary diseases, endocrine diseases, neurological diseases, tumors and other diseases, other pancreatic diseases, history of mental diseases.

Normal subjects and newly diagnosed male patients with type 1 or type 2 diabetes