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The purpose of the proposed project is to determine if short-term dietary supplementation of magnesium will improve performance during a series of lab-based exercise tests, will favorably modify the gut-microbiota, and will augment skeletal muscle mitochondrial function.
Magnesium is the fourth most abundant mineral in the human body. It is critical to day-to-day physiological function including the regulation of metabolism, cardiovascular function, immune function, and the operation of the nervous system. In light of its important role in physiology, dietary supplementation of magnesium has been purported to improve athletic performance, although the precise mechanism is unclear. The foci of the proposed study is the ergogenic effects of magnesium, its potential influence on gut health, and its potential ability to improve skeletal muscle function. The investigators will be studying an athletic/competitive population of endurance-trained adults. This group is likely to be the most interested in the use of magnesium to enhance athletic performance. Also, by only recruiting habitual exercisers, the variability between participants is likely to be reduced compared with if the investigators had also recruited people who are usually sedentary.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| ReMag | Experimental | Liquid Lemon flavor drink containing 300 mg magnesium chloride (Regmag) consumed twice daily for 9 days |
|
| ReMag Placebo | Placebo Comparator | Liquid Lemon flavor drink placebo comparator consumed twice daily for 9 days. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| ReMag | Dietary Supplement | 300 mg of ReMag dissolved in lemon flavor liquid |
|
| Measure | Description | Time Frame |
|---|---|---|
| Comparison of completion of time trial to placebo | Amount of time it takes to cycle 10 kilometers | After 9 days of intervention |
| Comparison of power output to placebo | Cycling for 30 seconds | After 9 days of intervention |
| Comparison of indirect Calorimetry (VO2max) to placebo | During a standard stress test VO2 max will be measured | After 8 days of intervention |
| Comparison of lactate threshold to placebo | During a standard stress test lactate threshold will be measured | After 8 days of intervention |
| Comparison of Mitochondrial Function to placebo | Assessed via high-resolution mitochondrial respirometry in permeabilized skeletal muscle. | After 8 days of intervention |
| Comparison of Shannon and Faith's microbiota diversity scores in feces to placebo | Assessed via 16s ribosomal ribonucleic acid microbial profiling | After 9 days of intervention, |
| Calculation and ordination of B-diversity scores for all fecal samples to assess clustering | Assessed via 16s ribosomal ribonucleic acid microbial profiling | After 9 days of intervention |
| Determination of differentially abundant microbiota in feces of collected during treatment compared to placebo |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Colorado State University, Dept. of Health and Exercise Science | Fort Collins | Colorado | 80523-1582 | United States |
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| ID | Term |
|---|---|
| D015636 | Magnesium Chloride |
| ID | Term |
|---|---|
| D002712 | Chlorides |
| D006851 | Hydrochloric Acid |
| D017606 | Chlorine Compounds |
| D007287 | Inorganic Chemicals |
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Participants will be randomized to either placebo for 9-days of the intervention separated by a 3-week washout period.
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Coded bottles
| Placebo | Dietary Supplement | Lemon flavored liquid |
|
Assessed via 16s ribosomal ribonucleic acid microbial profiling |
| After 9 days of intervention |
| Comparison of abundant microbiota to markers in feces to placebo | Assessed via Linear discriminant analysis Effect Size algorithm | After 9 days of intervention |
| Comparison Human Granulocyte Macrophage Colony-Stimulating Factor to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interferon gamma to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 1 beta to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 2 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 4 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 5 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 6 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 7 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 8 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 10 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 12 (p70) to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison interleukin 13 to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison Tumor Necrosis Factor alpha to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| Comparison High-sensitivity C-reactive protein to placebo | Assessed via 13-plex human T-cell cytokine panel | After 9 days of intervention |
| D017616 |
| Magnesium Compounds |