The body composition measurement was conducted using the Tanita BC-420 body composition analyzer, which collects data on body fat percentage. The participants stood on the foot electrodes of the analyzer and held the hand electrode firmly while maintaining a stable posture throughout the process. The measurement was completed using the bio-electrical impedance method, which typically took between 40 to 60 seconds to obtain accurate readings of the aforementioned body composition metrics.

The upper arm medical electronic sphygmomanometer is used to measure blood pressure. During the measurement, the cuff of the sphygmomanometer is securely wrapped around the upper arm at the level of the heart, ensuring a proper fit without constriction. The device is then activated to inflate the cuff, applying pressure to the brachial artery until the pulse is no longer detectable. The pressure is gradually released, and the device records the systolic pressure (the maximum pressure in the arteries during the heart's contraction) and the diastolic pressure (the minimum pressure in the arteries during the heart's relaxation).

Blood glucose analysis was conducted using a professional method to evaluate the blood sample. First, a skilled nurse performed venipuncture to collect the blood samples from the participants. The collected blood samples were immediately frozen and stored in a -80℃ freezer until all samples were gathered for unified delivery to the laboratory. The blood samples were sent to a specialized testing center for analysis and result feedback.

Total cholesterol analysis was conducted using a professional method to evaluate the blood sample. First, a skilled nurse performed venipuncture to collect the blood samples from the participants. The collected blood samples were immediately frozen and stored in a -80℃ freezer until all samples were gathered for unified delivery to the laboratory. The blood samples were sent to a specialized testing center for analysis and result feedback.

Low-density lipoprotein cholesterol (LDL-C) was conducted using a professional method to evaluate the blood sample. First, a skilled nurse performed venipuncture to collect the blood samples from the participants. The collected blood samples were immediately frozen and stored in a -80℃ freezer until all samples were gathered for unified delivery to the laboratory. The blood samples were sent to a specialized testing center for analysis and result feedback.

High density lipoprotein cholesterol (HDL-C) change was conducted using a professional method to evaluate the blood sample. First, a skilled nurse performed venipuncture to collect the blood samples from the participants. The collected blood samples were immediately frozen and stored in a -80℃ freezer until all samples were gathered for unified delivery to the laboratory. The blood samples were sent to a specialized testing center for analysis and result feedback.

Triglycerides was conducted using a professional method to evaluate the blood sample. First, a skilled nurse performed venipuncture to collect the blood samples from the participants. The collected blood samples were immediately frozen and stored in a -80℃ freezer until all samples were gathered for unified delivery to the laboratory. The blood samples were sent to a specialized testing center for analysis and result feedback.

Uric acid was conducted using a professional method to evaluate the blood sample. First, a skilled nurse performed venipuncture to collect the blood samples from the participants. The collected blood samples were immediately frozen and stored in a -80℃ freezer until all samples were gathered for unified delivery to the laboratory. The blood samples were sent to a specialized testing center for analysis and result feedback.

Professional salivary hormone detection methods were employed to analyze the saliva samples from participants. First, participants were instructed to collect saliva samples at a specified time point, typically upon waking in the morning. Collected saliva samples were stored in sterile containers and refrigerated promptly to prevent hormone degradation. Estradiol was measured from the saliva sample. The testing methods utilized high-sensitivity enzyme-linked immunosorbent assays (ELISA) to ensure precise and reliable results.

Use polypropylene urine cups and urine collection tubes (without PAEs plasticizers) to collect 10 mL of urine from the research subjects, and reserve the sample to collect the first excretion after getting up in the morning, before breakfast and before exercising. The collected urine samples were uniformly stored in a 5mL centrifuge tube and stored in a -20°C refrigerator. Urine PAEs and their metabolites: Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to detect seven PAEs metabolites in students' urine: monomethyl phthalate (MMP), phthalic acid Monoethyl ester (MEP), mono-n-butyl phthalate (MBP), mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-phthalate) Hydroxyhexyl) ester (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP).

To understand students' sleep duration, data collection was conducted using sleep diaries. Each participant was required to record at least one school day and one weekend day consecutively. The sleep diary primarily recorded bedtime, actual sleep onset time, and morning wake-up time. These data were used to calculate total sleep duration per night.

Put the photometer on the bedside table of the bedroom and program it to continuously collect light information every 60 seconds.

This is part of student questionnaire, which was filled by students or with the help of their parents. Basic information includes students' school, grade, class, name, gender, and date of birth.

This is part of student questionnaire, which was filled by students or with the help of their parents. The knowledge mastery part assessing health knowledge focus on whether students can distinguish behaviors include key message of '22510ss' that promote health. Responses were categorized as "Correct" "Incorrect" or "Don't Know" The total score is the sum of each item (0 to 7), "Correct" for 1 point, and "Incorrect" "Don't Know" for 0 point. A higher total score indicates a better understanding of health knowledge.

This is part of student questionnaire, which was filled by students or with the help of their parents. The health behavior section of the questionnaire surveys students on their screen time, consumption of fruits and vegetables, intake of high-quality protein, frequency of consuming sugary drinks, and frequency of vision and weight measurement. We calculate the score of '22510ss' key healthy behavior based on their questionnaire results. Each behavior earns one point for completion, and the total score for the items is calculated. The highest score is 7 points, and the lowest is 0 point. Higher points indicate stronger health action capabilities among students.

This is part of student questionnaire, which was filled by students or with the help of their parents. This section of the questionnaire investigates factors that promote or hinder students' implementation of healthy behaviors, including school support, family influence, and peer influence. A five-point Likert scale ("Strongly Agree" is scored 5 points, and "Strongly Disagree" is scored 1 point) is used, and if more than 75% of respondents choose "Agree" or "Strongly Agree," the factor is considered to have a strong promoting or hindering effect.

This is part of student questionnaire, which was filled by students or with the help of their parents. We used DQQ-China (Dietary Quality Questionnaire for China) to assess the dietary quality of student. DOQ collects dietary consumption data from 29 food groups and is used to calculate the Global Dietary Recommendation (GDR) score. The GDR score ranges from 0 to 18 and reflects adherence to the global dietary guidelines proposed by the World Health Organization (WHO). Higher GDR scores indicate better adherence to WHO's global dietary recommendations. Dietary quality classification based on the GDR score is as follows: High dietary quality: GDR ≥ 10; Moderate dietary quality: 8 ≤ GDR < 10; Low dietary quality: GDR < 8.

This is part of student questionnaire, which was filled by students or with the help of their parents. International Physical Activity Questionnaire (IPAQ) was used to assess physical activity levels, which includes the frequency, duration, and intensity of physical activities such as walking, moderate-intensity activities, and vigorous-intensity activities over the past 7 days, and the long form providing a more detailed assessment over a longer period. The total physical activity score was usually calculated in terms of MET-minutes/week (Metabolic Equivalent of Task), Based on the MET values (<600, 600-1500, >1500), students' activity levels are classified as low, moderate, or high.

This is part of student questionnaire, which was filled by students or with the help of their parents. The Center for Epidemiologic Studies Depression Scale (CES-D) was employed, each question on the MEQ-5 is answered on a scale that reflects the degree of preference for morning or evening activity. The scores from the five items are summed up to generate an overall score that reflects the individual's chronotype: The total score of the MEO-5 ranges from 4 to 25. The scoring categories are as follows: 4-7 points: Definitely Evening Type; 8-11 points: Moderately Evening Type; 12-17 points: Intermediate Type; 18-21 points: Moderately Morning Type;22-25 points: Definitely Morning Type.

This is part of student questionnaire, which was filled by students or with the help of their parents. The Center for Epidemiologic Studies Depression Scale (CES-D) was employed to assess the frequency and severity of depressive symptoms The CES-D consists of 20 items using a 4-point Likert scale (0 = "Rarely or none of the time," 3 = "Most or all of the time"). Scores for each item are summed, with higher total scores indicating more severe depressive symptoms. The total score ranges from 0 to 60. Generally, a score of 16 or higher is considered indicative of clinically significant depressive symptoms.

This is part of parents' questionnaire. Basic information includes their child's school, grade, class, name, gender, and date of birth, as well as their relationship, sibling information, birth information of the child, and the parents' age.

This is part of parents' questionnaire. This section surveys the height, weight, vision status, and the presence of diseases such as hypertension and diabetes in the father and mother.

This is part of parents' questionnaire. The knowledge mastery part assessing health knowledge focus on whether students can distinguish behaviors include key message of '22510ss' that promote health. Responses were categorized as "Correct" "Incorrect" or "Don't Know" The total score is the sum of each item (0 to 7), "Correct" for 1 point, and "Incorrect" "Don't Know" for 0 point. A higher total score indicates a better understanding of health knowledge.

This is part of parents' questionnaire. The health behavior section of the questionnaire surveys parents on their screen time, consumption of fruits and vegetables, intake of high-quality protein, frequency of consuming sugary drinks, and frequency of vision and weight measurement. We calculate the score of '22510ss' key healthy behavior based on their questionnaire results. Each behavior earns one point for completion, and the total score for the items is calculated. The highest score is 7 points, and the lowest is 0 point. Higher points indicate stronger health action capabilities among students.

This is part of parents' questionnaire. This section of the questionnaire surveys parents about their child's sleep performance using Sleep Disturbance Scale for Children (SDSC). The SDSC consists of 26 items that cover various aspects of sleep, categorized into different domains, it uses a 5-point Likert scale (1 = "never," 5 = "always"), where parents report how often their child experiences each symptom. The scores are summed to generate a total score and sub-scores for each domain. Total score range of 26 to 130, we used the following thresholds to classify the severity of sleep disturbances: Score < 38: Considered no significant sleep disturbance; Scores between 38 and 45: Mild sleep disturbance; Score > 45: Significant sleep disturbance.

This is part of school questionnaire. Basic information includes the school doctors' gender, age, education level, and workplace.

This is part of school questionnaire. We investigated the promoting and hindering factors in schools' efforts to improve students' health behaviors, including items such as the school's educational philosophy, school environment, and level of school support. The items are scored using a 5-point Likert scale ("Strongly Agree" is scored 5 points, and "Strongly Disagree" is scored 1 point). If more than 50% of school doctors select "Strongly Agree" for a particular item, it is considered an important promoting or hindering factor.