This study aims to detect the presence and diversity of Tissue resident memory T cell populations in early and late cases of generalized non-segmental Vitiligo.

• Written informed consent obtained from every participant.
• Detailed history taking including onset, course, duration of the disease, aggravating factors and received medications
• VASI score assessed in every patient.
• Patients photographed, divided into two groups early & late cases and 3 mm punch biopsies will be taken from perilesional and non lesional skin.
• Biopsies processed and stained for CD8 & CD69 in cases of early group, & late group and controls by immunofluorescence.

Inclusion Criteria:

• - Patients with Generalized non-segmental Vitiligo ( lesions in more than one anatomical site ).
• Both genders.
• Early group: onset less than or equal 6 months.
• Late group: onset more than 6 months

Exclusion Criteria:

• Localized or Segmental Vitiligo.
• Age < 14 years.
• Pregnant females
• Patient who received treatment of any type within the last 3 months for the late group.
• Patients with other skin diseases or immunosuppression

Generalized Non-segmental Vitiligo