Ovulatory dysfunction is identified in 15% of all infertile couples and it accounts for 40% of female infertility. Ovulatory dysfunction may be more subtle in women with regular menses. Detecting the day of ovulation is necessary for optimizing natural conception, diagnosis of cycle disturbances and also timing for embryo transfer in natural cycle frozen-thawed embryo transfers. In order to diagnose ovulatory dysfunction, ovulation physiology and change of reproductive hormones during the menstrual cycle should be understood. In the present study we aimed to evaluate serial serum hormonal cut-off levels referenced to ovulation in subfertile women with regular menses.

Inclusion Criteria:

• women that have regular menses (menstrual cycle length 21-35 days)
• women who will come visits regularly
• women that are trying to conceive more than 1 year if women age is less than 35 years or more than 6 months if women age is >35 years

Exclusion Criteria:

• women who do not menstruate regularly
• women that use hormonal contraception
• women who take drugs that will interfere with steroid hormone metabolism

The study population will be selected from women who attended to Acıbadem University Atakent Hospital Infertility Clinic for evaluation of infertility.