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The objectives of this study are to investigate the effect of fruit consumption on the gut microbiota and their collective fecal and plasma metabolomes, vascular and cardiometabolic functions, cognition, and motor control.
The Investigators findings will provide data critical to the understanding of the effects of fruit intake on the gut microbiome and the capacity of the microbiota to biotransform nutrients contained within fruits as well as the subsequent impact on circulating status of those metabolites. Concurrently, health outcomes that may be linked to these changes including cognition, motor control, vascular function and cardiometabolic health risk markers will be assessed in healthy adults.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Fruits | Experimental | Participants will receive 2 cup eq fruits per day |
|
| Fruit restriction | Other | Participants will receive 1/2 cup eq fruits per day |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Fruit (2 cup eq) | Other | participants will receive 2 cup eq fruits |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Change from baseline in composition of bacterial fecal microbiome at 8 weeks | Bacterial fecal microbiome 16S rRNA gene amplicon libraries will be sequence using the Illumina MiSeq next generation sequencing platform. | At baseline, at 8 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| Change from baseline in processing speed (cognition) at 8 weeks | using Pattern Comparison Processing Speed Test | At baseline, at 8 weeks |
| Change from baseline in Vascular health at 8 weeks | To better understand how changes in the gut microbiota through fruit consumption are linked to changes in health outcomes, we will assess endothelial function via flow-mediated vasodilation and risk factors for cardiometabolic dysfunction in fasting blood samples collected at baseline and after 8 weeks. |
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Inclusion Criteria:
Exclusion Criteria:
History of:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Shirin Hooshmand | San Diego | California | 92182-7251 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 41081007 | Derived | Zingales S, Martinez J, Stepp AH, Miller C, Jason N, Hong MY, Liu C, Kern M, Hooshmand S. Daily Intake of Recommended Servings of Fruit Improves Nutrient Intake but Shows no Major - Effect on Cardiovascular Health or Cognition in Low Fruit Consumers. Curr Dev Nutr. 2025 Sep 3;9(10):107544. doi: 10.1016/j.cdnut.2025.107544. eCollection 2025 Oct. |
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| ID | Term |
|---|---|
| D005638 | Fruit |
| ID | Term |
|---|---|
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D019602 | Food and Beverages |
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| Fruit restriction (1/2 cup eq) |
| Other |
participants will receive 1/2 cups eq fruits |
|
| At baseline, at 8 weeks |