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| Name | Class |
|---|---|
| Institut national de la recherche scientifique | UNKNOWN |
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This study aims to better understand the effects of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, virus that causes the coronavirus disease 2019 (COVID-19), on male fertility.
After being informed about the study and potential risks, all patients giving written informed consent will produce a sperm sample by masturbation. Patients will also complete a questionnaire relating to their state of health. Six month following the first sperm sample, patients will produce another sperm sample by masturbation. These sperm parameters will be compared with the ones from a sperm analysis performed less than 2 years before the SARS-CoV-2 infection.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| COVID-19 group | men aged 18 to 45 who have been infected with SARS-CoV-2 in the past 6 months |
| |
| Control group | healthy men aged 18 to 45 with normal sperm parameters (WHO 2010 criteria) and who have never contracted COVID-19 |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sperm analysis | Diagnostic Test | Routine sperm analysis including analysis of sperm concentration, motility, vitality and morphology. Sperm DNA integrity will also be assessed. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Changes in sperm parameters quality | Sperm concentration and motility will be assessed using computer-assisted sperm analysis (CASA). Sperm vitality and morphology will be evaluated using eosine-nigrosine and Romanowsky staining techniques, respectively. DNA fragmentation and sperm chromatin decondensation will be measured using the Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and aniline blue assays, respectively. These sperm parameters will be compared before and after SARS-CoV-2 infection and between the different time frames. | Pre-infection, on the day of recruitment and six month later |
| Viral RNA in seminal plasma | The number of SARS-CoV-2 RNA copies and viral particles / mL of seminal plasma will be assessed | Pre-infection, on the day of recruitment and six month later |
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Inclusion Criteria:
Exclusion Criteria:
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See Eligibility Criteria
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Fertilys | Laval | Quebec | H2P2L6 | Canada |
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| ID | Term |
|---|---|
| D000086382 | COVID-19 |
| D007248 | Infertility, Male |
| ID | Term |
|---|---|
| D011024 | Pneumonia, Viral |
| D011014 | Pneumonia |
| D012141 | Respiratory Tract Infections |
| D007239 | Infections |
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| D014777 |
| Virus Diseases |
| D018352 | Coronavirus Infections |
| D003333 | Coronaviridae Infections |
| D030341 | Nidovirales Infections |
| D012327 | RNA Virus Infections |
| D008171 | Lung Diseases |
| D012140 | Respiratory Tract Diseases |
| D005832 | Genital Diseases, Male |
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
| D007246 | Infertility |
| D052801 | Male Urogenital Diseases |