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| Name | Class |
|---|---|
| Universiteit Antwerpen | OTHER |
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The goal of this study is to optimize two methods for microbiome analysis of skin and upper airway samples (16S amplicon sequencing and Shotgun sequencing). In addition, the researchers will investigate whether there is an effect of wearing a face mask on the microbial communities of the skin and upper airways. The volunteers will self-collect swabs of the skin and upper airways (nose and/or throat). Bacterial DNA and/or total RNA will be isolated and the microbial communities will be investigated with 16S amplicon sequencing and shotgun metagenomic sequencing.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| No face mask | Volunteers who did not wear a face mask |
| |
| Cotton face mask | Voluntarily wearing a cotton face mask for 4 hours, following the government's guidelines during the COVID-19 pandemic. |
| |
| Surgical face mask | Voluntarily wearing a surgical face mask for 4 hours, following the government's guidelines during the COVID-19 pandemic. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Observational intervention | Other | Voluntarily (not) wearing a face mask (cotton or surgical) for 4 hours, following the government's guidelines during the COVID-19 pandemic. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Change from baseline in microbiome composition of the skin and upper airways after wearing a face mask | 16S rRNA sequencing | Start, 4 hours |
| Optimalization of microbiome analysis of skin and upper airway samples | 16S rRNA sequencing, shotgun metagenomic sequencing | Start |
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Inclusion Criteria:
Exclusion Criteria:
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Healthy, adult volunteers
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Sarah Lebeer, Prof. | Contact | +3232653285 | sarah.lebeer@uantwerpen.be | |
| Veronique Verhoeven, Prof. Dr. | Contact | +3232652518 | veronique.verhoeven@uantwerpen.be |
| Name | Affiliation | Role |
|---|---|---|
| Sarah Lebeer, Prof. | Universiteit Antwerpen | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Antwerp and University Hospital Antwerp | Recruiting | Antwerp | 2020 | Belgium |
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Isolation of microbial DNA and/or RNA out of skin and upper airway swabs.