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Investigation of the vitreoretinal contact microarchitecture in the macular and paramacular zones of the human retina.
Purpose: to study the features of the macular ILM, to study the features of the preretinal structures of the vitreous body of the Central retinal region, morphofunctional features of preretinal hyalocytes, and the mechanism of vitreoretinal adhesion.
Study Protocol: examination of donor human eyes obtained from an eye Bank after sampling material for corneal transplantation.
Research method: scanning electron microscopy (SEM) of the central retina (macular and paramacular zone): ILM, preretinal vitreous.
Scientific hypothesis: vitreoretinal adhesion is provided not by laminin and fibronectin, but by the interweaving of vitreous collagen fibers between the epiretinal vitreous (more firmly fixed to the retinal surface) and the cortical vitreous.
Expected results: determination of ILM thickness in the macular zone, microanatomy of preretinal vitreous strutcures, and possible mechanism of vitreoretinal adhesion, establishing the possibility of intravital synthesis of collagen fibers synthesis in the adult vitreous.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| cadaver eyes | Experimental | The Central region of the retina and preretinal structures of cadaver eyes are examined |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| scanning electron microscopy | Other | scanning electron microscopy of the сentral retina and preretinal structures |
|
| Measure | Description | Time Frame |
|---|---|---|
| the ILM thickness in the macular zone (foveola) | the ILM thickness in the macular zone (foveola) in mkm by scanning electron microscopy data | within 3 days after receiving the cadaver eyes |
| superficial microstructure of ILM | by scanning electron microscopy data | within 3 days after receiving the cadaver eyes |
| features of the structural microarchitecture of the preretinal vitreous in the macular zone | by scanning electron microscopy data | within 3 days after receiving the cadaver eyes |
| morphofunctional features of preretinal hyalocytes. | by scanning electron microscopy data | within 3 days after receiving the cadaver eyes |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Pavel V Lyskin, PhD | The S.N. Fyodorov Eye Microsurgery State Institution | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| S.N. Fedorov Nmrc "Mntk "Eye Microsurgery" | Moscow | 127486 | Russia |
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| Label | URL |
|---|---|
| Microanatomy of vitreoretinal interactions in macular and paramacular areas (article DOI: 10.17816/KMJ2019-595) | View source |
| Microanatomy of Internal Limiting Membrane (article) | View source |
| Cellular Microanatomy of Vitreoretinal Interface. Morphofunctional Identification of Epiretinal Vitreous Cells. Collagen Synthesis in Adult Human Vitreous (article) |
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| ID | Term |
|---|---|
| D008855 | Microscopy, Electron, Scanning |
| ID | Term |
|---|---|
| D008854 | Microscopy, Electron |
| D008853 | Microscopy |
| D003952 | Diagnostic Imaging |
| D019937 | Diagnostic Techniques and Procedures |
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| View source |
| New Data of Mechanism in Vitreoretinal Adhesion and Posterior Vitreous Detachment in Humans (article) | View source |
| Antithesis to the thesis about fibronectin and laminin as the main components providing vitreoretinal adhesion (article) | View source |
| D003933 | Diagnosis |
| D008919 | Investigative Techniques |