The purpose of this study is to describe the use of methods confirming brain death in the real clinical practice of the transplant program in the Czech Republic.

Organ donation is possible in the Czech Republic only after meeting the legislative requirements. These require confirmation of brain death by one of the statutory methods before organ harvesting. The use of these methods in clinical practice has not yet been described.The aim of this retrospective observational study is to describe the use of these methods in the real clinical practice of the transplant program in the Czech Republic, taking into account the legislation of other European countries.

Inclusion Criteria:

• age ≥18 years
• organ donor

Exclusion Criteria:

• age under 18 years

Adult critically ill patinets with confirmed brain death admitted to university hospital who become brain death organ donor.