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The proposed research is an intervention and feasibility pilot trial designed to determine if short-term consumption of healthy cookies made with an oil rich in linoleic acid can influence mitochondria biology in white blood cells and muscle. The hypothesis of the study is that consuming 1 healthy cookie rich in linoleic acid each day for two weeks will improve mitochondrial biology in white blood cells and muscle.
Linoleic acid has many health benefits including altering body composition and energy metabolism, possibly through its impact on cardiolipin and mitochondria.
The investigators plan to test the central hypothesis and accomplish the overall objective of this research by pursuing the following four specific aims
Aim 1) To assess muscle biopsy yield with a micro biopsy needle
Aim 2) To determine the amount of peripheral blood mononuclear cells (PBMC) and muscle needed to measure mitochondria function
Aim 3) To assess the feasibility of measuring mitochondria function on fresh PBMC and muscle samples
Aim 4) To determine the effect of short-term healthy cookies made with linoleic acid rich oil on measures of mitochondrial function in white blood cells and muscle as well as their relationship to lipid composition of the blood, markers of metabolic health and markers of mitochondria biogenesis
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy Cookie Group | Experimental | All participants in the study |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High Linoleic Acid Healthy Cookies | Other | High Linoleic Acid Healthy Cookies (containing about 10g of grapeseed oil) 1 per day for 2 weeks |
|
| Measure | Description | Time Frame |
|---|---|---|
| Changes in mitochondria function | Mitochondria function measured in peripheral blood mononuclear cells and skeletal muscle | Week 0 to Week 2 |
| Changes in cardiolipin species | Cardiolipin species in peripheral blood mononuclear cells and skeletal muscle | Week 0 to week 2 |
| Correlation of mitochondrial biology in blood and skeletal muscle | Correlation of mitochondria function and cardiolipin species in peripheral blood mononuclear cells and skeletal muscle | Week 0 and week 2 |
| Measure | Description | Time Frame |
|---|---|---|
| Changes in fasting fatty acid levels | Linoleic acid content of fasting blood samples | Week 0 to week 2 |
| Correlation of fatty acids with mitochondrial function and cardiolipin species | Correlation of linoleic acid in blood with mitochondria function and cardiolipin species in peripheral blood mononuclear cells and skeletal muscle |
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Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| The Ohio State University | Columbus | Ohio | 43210 | United States |
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| Week 0 and week 2 |
| Correlation of cardiolipin with global sleep quality score, Cambridge physical activity index and dietary intake of omega-3 fats | Correlation of cardiolipin in peripheral blood mononuclear cells and skeletal muscle with measurements of sleep quality using the Pittsburgh Sleep Quality Index, physical activity using the Epic Physical activity short questionnaire and dietary omega-3 intake using the Omega-3 Checklist | Week 0 and week 2 |
| Correlation of cardiolipin with body mass index, sagittal diameter (abdominal thickness), walking speed and grip strength | Correlation of cardiolipin in peripheral blood mononuclear cells and skeletal muscle with measurements of body mass index, sagittal diameter, grip strength and gait speed | Week 0 and week 2 |