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Irisin is a myocin secreted by skeletal muscle in stimulus to physical exercise and has been described as a possible therapeutic tool in the fight against obesity by triggering a cascade of signaling that triggers the expression of genes responsible for the increase in energy expenditure and browning of adipose tissue white cells by activating thermogenesis promoter mitochondrial proteins. Although the science is engaged in the search for evidences about the actions of the irisin, the physical exercise involved and the improvement in the levels of obesity, many mechanisms are still unknown. Since taurine by means of irisin-like pathways also increases energy expenditure, it is believed that taurine supplementation associated with high intensity aerobic physical exercise can optimize the effects of irisin, increasing energy expenditure and improving body composition in obesity.
Introduction: Irisin is a myocin secreted by skeletal muscle when stimulated by physical exercise that promotes increased thermogenesis (browning effect). Similarly, taurine is also associated with modulations of energy metabolism with promising actions in the control of metabolic disorders. Thus, it is believed that chronic taurine supplementation associated with physical training can optimize irisin concentrations after exercise.
Objective: Evaluate the effects of taurine supplementation associated with interval aerobic training on plasma irisin concentrations, energy expenditure, body composition and physical fitness in obese women. Methods: Twenty-two women aged 25 to 45 years, sedentary, with grade I obesity (BMI ≥30-35 kg / m²) and without comorbidities will be recruited for the study. The subjects will be submited to a high intensity interval aerobic training for 8 weeks, being 3 times by week, for 50 minutes, associated to the supplementation with 3g of taurine or placebo.
Methods: Assessment of dietary intake, Resting Energy Expenditure (GER) by indirect calorimetry, body composition by deuterium oxide, anthropometric measurements, HPLC plasma analysis of taurine, multiplex irisin and physical fitness variables will be performed pre and post intervention. The results will be expressed as mean and standard deviation and the ANOVA test two way repeated measures mixed model will be applied, with post hoc Sidak, to verify differences and statistical interactions (p <0.05).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Taurine group and exercise | Active Comparator | Intervention with taurine supplementation and physical training. |
|
| Placebo group and exercise | Placebo Comparator | Intervention with placebo supplementation and physical training. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Taurine group and exercise and placebo group and exercise | Dietary Supplement | Both groups supplemented with taurine and / or placebo will perform physical training. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Changes body weight | The body weight was measured by digital balance before and after the intervention | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| Changes body composition | The body composition was evaluated through deuterium oxide | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| Changes indirect calorimetry | With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (lipids and carbohydrates) | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| Changes in total cholesterol and lipid fractions | Collected in EDTA tubes, were centrifuged. | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| Changes in plasma taurine concentration | Collected in EDTA tubes, were centrifuged and the plasma taurine concentration was evaluated using HPLC (high performance liquid chromatography). | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| Changes in plasma irisin concentration | Collected in EDTA tubes, were centrifuged and the plasma irisin concentration was evaluated using Multiplex - Kit Human Myokine Magnetic Bead Panel. | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Gabriela Batitucci | Ribeirão Preto | São Paulo | Brazil |
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| ID | Term |
|---|---|
| D009765 | Obesity |
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D015444 | Exercise |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
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double blind trial
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Intervention with taurine and / or placebo supplementation and physical training
| Physical training with Deep Water Running | Other | There will be a physical training for eight weeks. |
|
| Changes Food intake | Food registry of 3 days. The quantification of the daily intake of nutrients will still be made using sofware. | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| Changes in determination of lactate | Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser. | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| Changes Physical Performance | Maximum effort test, according to Wilder, Brennan e Schotte (1993). | Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D001519 | Behavior |