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| ID | Type | Description | Link |
|---|---|---|---|
| 2020-A00593-36 | Other Identifier | ID-RCB |
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septic shock is the first cause of mortality in intensive care unit. Innate immunity is the body's first line of defense against pathogens
since their discovery, inflammasomes have an important role during inflammatory response following an aggression. There are intracytoplasmic multiprotein complex activated by cellular stress or infections and is responsible for the release of pro-inflammatory cytokines, including IL-1β. One study focused on the role of the NLRP3 inflammasome in monocyte during sepsis has shown its early alteration that is correlated with mortality. Most studies have analysed inflammasomes in nucleated cells, nevertheless, little is known about inflammasomes in platelets. The aim of the study is to analyze the activation of NLRP3 inflammasome in platelets and leukocytes during sepsis, from patients hospitalized in intensive care unit compare to a control group, until its resolution to better understand the pathophysiology of sepsis
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sepsis group | patients suffering from sepsis hospitalized in intensive care unit |
| |
| Control group | patients without infection hospitalized in intensive care unit |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood collection | Other | Blood collection (at admission, two days, one week and at discharge) to analyze the activation of NLRP3 inflammasome in platelets and leukocytes |
|
| Measure | Description | Time Frame |
|---|---|---|
| Activation kinetics of the NLRP3 inflammasome during sepsis until its resolution | Activation kinetics of the NLRP3 inflammasome from day 0 to patient discharge measured by the area under curve | From day 0 to the hospital discharge, an average of 1 month |
| Measure | Description | Time Frame |
|---|---|---|
| Rate of inflammasome NLRP3 activation | Rate of inflammasome NLRP3 activation (measured in blood samples) | Day 0 |
| Rate of inflammasome NLRP3 activation | Rate of inflammasome NLRP3 activation (measured in blood samples) |
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SEPSIS GROUP:
Inclusion Criteria:
Exclusion Criteria:
CONTROL GROUP:
Inclusion Criteria:
Exclusion Criteria:
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Patients hospitalized in intensive care unit in University Hospital of Toulouse
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| Name | Affiliation | Role |
|---|---|---|
| Fanny BOUNES, PH | University Hospital, Toulouse | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Hospital of Toulouse | Toulouse | France | 31100 | France |
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| ID | Term |
|---|---|
| D018805 | Sepsis |
| D013927 | Thrombosis |
| D012772 | Shock, Septic |
| ID | Term |
|---|---|
| D007239 | Infections |
| D018746 | Systemic Inflammatory Response Syndrome |
| D007249 | Inflammation |
| D010335 | Pathologic Processes |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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Blood samples will be collected in peripheral blood at the admission in intensive care unit, day two, day seven and at discharge to compare inflammasome NLRP3 and others markers during sepsis and after resolution of the disease.
| Day 2 |
| Rate of inflammasome NLRP3 activation | Rate of inflammasome NLRP3 activation (measured in blood samples) | Day 7 |
| Rate of inflammasome NLRP3 activation | Rate of inflammasome NLRP3 activation (measured in blood samples) | Day of hospital discharge, an average of 1 month |
| Severity score measured by Sequential Organ Failure Assessment (SOFA) score | Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome | Day 0 |
| Severity score measured by Sequential Organ Failure Assessment (SOFA) score | Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome | Day 2 |
| Severity score measured by Sequential Organ Failure Assessment (SOFA) score | Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome | Day 7 |
| Severity score measured by Sequential Organ Failure Assessment (SOFA) score | Severity score measured by Sequential Organ Failure Assessment (SOFA) score, from 0 to 4; higher score mean worse outcome | Day of hospital discharge, an average of 1 month |
| Severity score measured by Simplified Gravity Index 2 | Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome | Day 0 |
| Severity score measured by Simplified Gravity Index 2 | Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome | Day 2 |
| Severity score measured by Simplified Gravity Index 2 | Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome | Day 7 |
| Severity score measured by Simplified Gravity Index 2 | Severity score measured by Simplified Gravity Index 2, score from 0 to 163, higher score mean worse outcome | Day of hospital discharge, an average of 1 month |
| D013568 |
| Pathological Conditions, Signs and Symptoms |
| D016769 | Embolism and Thrombosis |
| D014652 | Vascular Diseases |
| D002318 | Cardiovascular Diseases |
| D012769 | Shock |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |