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| ID | Type | Description | Link |
|---|---|---|---|
| DRKS00022950 | Registry Identifier | DRKS |
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| Name | Class |
|---|---|
| Helmholtz Institut für Metabolismus-, Adipositas- und Gefäßforschung | UNKNOWN |
| Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde | UNKNOWN |
| Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie |
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The sense of taste is essential for priming food preferences and is therefore important for caloric uptake and body weight regulation. Recent studies show that obesity is linked to changes in taste sensation but also to a loss of taste buds on the tongue. This study aims to evaluate underlying mechanisms within the taste buds to potentially influence the sense of taste on the level of these sensory cells and in order to develop new treatment strategies to fight obesity.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| normal weight | Normal weight adults with BMI ranging from 18.5-24.9; men and women; age range 18-69years; written informed consent |
| |
| obese | Subjects with obesity as defined by BMI >= 30; men and women; age range 18-69 years; written informed consent |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| taste bud biopsy | Procedure | Taste buds will be surgically removed a) during a planned and medically induced elective surgery under full anesthesia; b) by local anesthesia in subjects without a medically induced surgery |
| Measure | Description | Time Frame |
|---|---|---|
| Identification of differential gene and protein regulation in isolated human taste cells between lean and obese subjects | Transcriptomics (RNAsequencing (seq), single cell seq) and epigenomics (open chromatin mapping (ATACseq), Infinium MethylationEPIC Array) will be performed in isolated papillae fungiformes. Validation analyses by in vitro primary cell cultures of taste cell biopsy | through study completion, an average of 2 years |
| Identification of correlations between taste cell biology with parameters of intervention | corrleation analyses will include factors of eating behavior, food preferences, life style factors, data from taste and smell screenings, anthropometric data, blood and saliva parameters | 1 year |
| Measure | Description | Time Frame |
|---|---|---|
| Differences of salivary and tongue microbiome on taste cells in obesity in contrast to lean | whole mouth saliva as well as local tongue microbiome diversity will be analysed by 16S ribosomal RNA seq and related to further parameters of this study. | 2 years |
| extracellular vesicle analyses of saliva in lean and obese subjects |
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Inclusion Criteria:
Exclusion Criteria:
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the study population will mainly consits of patients who underwent a medically induced elective surgery at the head or neck area (f.e. surgery at the soft palate, plastic surgery, maintenance of congenital anomalies etc.) or healthy participants recruited elsewhere at the campus area of the Medical Faculty of the University of Leipzig, Saxony, Germany.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Imke Schamarek, Dr. | Contact | +493419713803 | Imke.Schamarek@medizin.uni-leipzig.de | |
| Kerstin Rohde-Zimmermann, Dr. | Contact | +493419722932 | Kerstin.Rohde@helmholtz-muenchen.de |
| Name | Affiliation | Role |
|---|---|---|
| Michael Stumvoll, Prof. Dr. | Medical Faculty of the University of Leipzig, Leipzig, Germany | Study Director |
| Imke Schamarek, Dr. | Medical Faculty of the University of Leipzig, Leipzig, Germany | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Medical Clinic III, Division of Endocrinology, University Clinic of Leipzig | Recruiting | Leipzig | Saxony | 04103 | Germany |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 38618969 | Derived | Kersten A, Lorenz A, Nottmeier C, Schmidt M, Roesner A, Richter FC, Rohrborn K, Witte AV, Hahnel S, Koehne T, Bluher M, Stumvoll M, Rohde-Zimmermann K, Schamarek I. The Obese Taste Bud study: Objectives and study design. Diabetes Obes Metab. 2024 Jun;26(6):2054-2068. doi: 10.1111/dom.15563. Epub 2024 Apr 15. |
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one aim of this study is to obtain large-scale sequencing data. with this several not yet defined research questions will be generated which shall be explored through later analyses and projects. in order to exploit the potential of the data and to avoid misinterpretation, the data should only be accessible to the scientists and collaborators involved.
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| ID | Term |
|---|---|
| D009765 | Obesity |
| D005518 | Food Preferences |
| D013651 | Taste Disorders |
| D008659 | Metabolic Diseases |
| D005247 | Feeding Behavior |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D005951 | Glucose Tolerance Test |
| D000886 | Anthropometry |
| D011795 | Surveys and Questionnaires |
| ID | Term |
|---|---|
| D001774 | Blood Chemical Analysis |
| D019963 | Clinical Chemistry Tests |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
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| Poliklinik für Zahnärztliche Prothetik und Werkstoffkunde | UNKNOWN |
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Taste buds, saliva, blood
| oral glucose tolerance test | Diagnostic Test | 75g glucose will be applied in drinking water following blood sample collection to evaluate glucose, insulin and C-peptide level. |
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| Taste sensitivity | Diagnostic Test | Subjects are given taste strips with 4 concentrations per each taste quality of sweet, sour, salty, bitter and umami in a pseudo-randomized manner. Subjects are asked to identify the taste quality, state the intensity, preference and certainty. |
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| olfactory function | Diagnostic Test | Subjects will be presented 16 different sniffing sticks in a randomized order, each for about 4seconds. People are then asked to identify the smell by choosing one out of 4 options (forced choice). |
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| taste bud density | Diagnostic Test | The number of taste buds on the anterior tongue will be determined by colouring the tongue surface with food colour. Taste buds will apply more reddish as compared to non-taste tissue. Based on a digital photography the number of taste buds per subjects is counted manually. |
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| anthropometry | Diagnostic Test | Anthropometric data collection will include measures of body height, body weight, arm-, upper- and lower leg circumferences, waist- and hip circumferences and a bioimpedance analysis to obtain data about body composition. |
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| biospecimen collection | Other | Blood and saliva samples will be taken as well as a tongue swab. |
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| questionnaires | Other | Through the use of several questionnaires, data about eating behavior, food preferences, life style factors and other will be obtained. |
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The content of extracellular vesicles from saliva samples will be analysed and compared between the study groups as well as related to other parameters of this study. |
| 2 years |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D001519 | Behavior |
| D012678 | Sensation Disorders |
| D009461 | Neurologic Manifestations |
| D009422 | Nervous System Diseases |
| D001522 | Behavior, Animal |
| D003933 | Diagnosis |
| D003940 | Diagnostic Techniques, Endocrine |
| D008919 | Investigative Techniques |
| D010808 | Physical Examination |
| D001699 | Biometry |
| D015991 | Epidemiologic Measurements |
| D011634 | Public Health |
| D004778 | Environment and Public Health |
| D003625 | Data Collection |
| D004812 | Epidemiologic Methods |
| D017531 | Health Care Evaluation Mechanisms |
| D011787 | Quality of Health Care |
| D017530 | Health Care Quality, Access, and Evaluation |