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The function of the autonomic nervous system can be assessed using baroreflex sensitivity (BRS) and heart rate variability (HRV). Decreased HRV has been shown to be predictive of morbidity and mortality in diverse medical conditions such as acute myocardial infarction, aneurysmal subarachnoid haemorrhage, autoimmune diseases, sepsis and surgery.
The function of the autonomic nervous system has not yet been investigated in a "pure hypovolemia" model. The aim of the current study is therefore to investigate and describe the function of the autonomic nervous system prior to, during and after reduction of blood volume in healthy blood donors.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Blood donors | Healhy young male bloddonors, aged 30-45 |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Monitoring of the autonomic nervous system | Device | Monitoring of the autonomic nervous system using E-patch, LiDCO and Root-Massimo. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Changes in root mean square of successive NN-interval differences (RMSSD) during mobilisation | Measured in ms | 15 minutes prior to and 15 minutes after blood donation |
| Measure | Description | Time Frame |
|---|---|---|
| Changes in root mean square of successive NN-interval differences (RMSSD) during sleep | Measured in ms | The night before and night after blood donation |
| Changes in standard deviation of N-N intervals (SDNN) during mobilisation |
| Measure | Description | Time Frame |
|---|---|---|
| Pain score | Measured by verbal rating scale (VRS) 0 - 10 (0 = no pain, 10 = worse pain imaginable) during mobilisation procedure | 15 minutes prior to and 15 minutes after blood donation |
Inclusion Criteria:
Exclusion Criteria:
Volonteers not eligable for blood donation following the Danish legislation, among others due to:
Furthermore:
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Healthy male volonteers, aged 30-45, that are eligable for blood donation following the Danish legislation
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| Name | Affiliation | Role |
|---|---|---|
| Henrik Kehlet, Professor | Rigshospitalet, Denmark | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Hvidovre University Hospital | Copenhagen | 2960 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 37752379 | Derived | Hristovska AM, Uldall-Hansen B, Mehlsen J, Andersen LB, Kehlet H, Foss NB. Orthostatic intolerance after acute mild hypovolemia: incidence, pathophysiologic hemodynamics, and heart-rate variability analysis-a prospective observational cohort study. Can J Anaesth. 2023 Oct;70(10):1587-1599. doi: 10.1007/s12630-023-02556-6. Epub 2023 Sep 26. |
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| ID | Term |
|---|---|
| D054969 | Primary Dysautonomias |
| D006470 | Hemorrhage |
| ID | Term |
|---|---|
| D001342 | Autonomic Nervous System Diseases |
| D009422 | Nervous System Diseases |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
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10 mL of blood stored in a biobank
Measured in ms
| 15 minutes prior to and 15 minutes after blood donation |
| Changes in standard deviation of N-N intervals (SDNN) during sleep | Measured in ms | The night before and night after blood donation |
| Percentage of successive RR intervals that differ by more than 50 ms (pNN50) during mobilisation | Measured in % | 15 minutes prior to and 15 minutes after blood donation |
| Percentage of successive RR intervals that differ by more than 50 ms (pNN50) during sleep | Measured in % | The night before and night after blood donation |
| Changes in low frequency (LF) power during mobilisation | Measured in ms^2 | 15 minutes prior to and 15 minutes after blood donation |
| Changes in low frequency (LF) power during sleep | Measured in ms^2 | The night before and night after blood donation |
| Changes high frequency (HF) power during mobilisation | Measured in ms^2 | 15 minutes prior to and 15 minutes after blood donation |
| Changes high frequency (HF) power during sleep | Measured in ms^2 | The night before and night after blood donation |
| Changes in ratio of LF-to-HF power during mobilisation | Measured in % | 15 minutes prior to and 15 minutes after blood donation |
| Changes in ratio of LF-to-HF power during sleep | Measured in % | The night before and night after blood donation |
| Changes in S (area of the ellipse which represents total HRV) during mobilisation | Measured in ms | 15 minutes prior to and 15 minutes after blood donation |
| Changes in S (area of the ellipse which represents total HRV) during sleep | Measured in ms | The night before and night after blood donation |
| Changes in SD1 (Poincaré plot standard deviation perpendicular the line of identity) during mobilisation | Measured in ms | 15 minutes prior to and 15 minutes after blood donation |
| Changes in SD1 (Poincaré plot standard deviation perpendicular the line of identity) during sleep | Measured in ms | The night before and night after blood donation |
| Changes in SD2 (Poincaré plot standard deviation along the line of identity) during mobilisation | Measured in ms | 15 minutes prior to and 15 minutes after blood donation |
| Changes in SD2 (Poincaré plot standard deviation along the line of identity) during sleep | Measured in ms | The night before and night after blood donation |
| Changes in SD1/SD2 ratio during mobilisation | Measured in ms | 15 minutes prior to and 15 minutes after blood donation |
| Changes in SD1/SD2 ratio during sleep | Measured in ms | The night before and night after blood donation |
| Changes in systolic arterial pressure (SAP) during mobilization | Measured in mmHg by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in diastolic arterial pressure (DAP) during mobilization | Measured in mmHg by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in mean arterial pressure (MAP) during mobilization | Measured in mmHg by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in pulse pressure (PP) during mobilization | Measured in mmHg by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in heart rate (HR) during mobilisation | Measured in beats min-1 by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in cardiac output (CO) during mobilization | Measured in L/min by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in stroke volume (SV) during mobilization | Measured in mL by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in systemic vascular resistance (SVR) during mobilization | Measured in dynes s cm-5 by non-invasive LiDCO | 15 minutes prior to and 15 minutes after blood donation |
| Changes in cerebral perfusion (ScO2) during mobilization | Measured in % by Root Masimo | 15 minutes prior to and 15 minutes after blood donation |
| Changes in muscular perfusion (SmO2) during mobilization | Measured in % by Root Masimo | 15 minutes prior to and 15 minutes after blood donation |
| Changes in peripheral index during mobilization | Measured in % by Root Masimo | 15 minutes prior to and 15 minutes after blood donation |
| Concentration of C-reacitve protein | Measured in gr/dL | At time of blood donation |
| V-PLEX Biomarker 54.Plex Kit | 54 key analytes that are important in inflammation response and immune system regulation as well as numerous other biological processes | Blood taken at time of blood donation |