PGTai Results for Embryos Cultured in Sequential Versus Single Step Media

Sequential Versus Single Step Culture Media in Relation to Euploidy Rates in PGTai Program

A prospective cohort study undergoing ICSI at a private fertility center in Egypt

Purpose:

To evaluate PGTai results for embryos cultured in sequential versus single step media.

Method:

Injected oocytes will divided between Continuous Single Culture-NX media (CSC- NX) with 10% SSS the first group (Irvine, USA) and the other group will be cultured in sequential cleavage and blastocyst media with 10% SPS (SAGE, Denmark).

All blastocytes with 4BB quality and better according to Gardner's criteria 1999 will go through PGTA procedures with PGTai confirmation.

Inclusion Criteria:

• Female age ≤37 years with ≥ 8 mature oocytes
• Male partner had normal semen parameters according to WHO 2010
• PGT-A was done at the blastocyst stage

Exclusion Criteria:

• sperm or oocyte donor
• severe male factors according to WHO 2010