Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
The aim of this study is to analyze whether there is difference in the effect of single embryo culture and group culture in preimplantation genetic screening (PGS) cycle, and provide reference for the feasibility of the clinical application of medium-based noninvasive PGD. This prospective study prepares for more than 1,000 normal fertilized zygotes in each group over one year period. For the recruited patients, all normal fertilized zygotes were randomly allocated into two groups: cultured individually in 25 ul media or 2-3 embryos cultured together in 50 ul media. The blastocyst formation rates, aneuploid rates, clinical pregnancy rates, implantation rates and miscarriage rates will be compared between the two groups.
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Single embryo culture | Other | Embryo is cultured individually in 25 ul media |
|
| Group embryo culture | Other | 2-3 Embryos are group cultured in 50 ul media |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| embryo culture | Other | Single embryo culture means only one embryo is cultured in one drop, and the group culture refers to culture of 2-5 embryos in one drop. The main advantage of group culture may be secreting some paracrine cytokines by embryos and benefit each other; and the possible disadvantage of group culture is that embryo secreting of some harmful substances which accumulated to a certain extent will damage the developmental potential of embryos. The advantages and disadvantages of single embryo culture are contrary to group culture. |
| Measure | Description | Time Frame |
|---|---|---|
| high quality blastocyst formation rate | number of blastocysts with high quality divided by number of zygotes with normal fertilization | 6 days after fertilization |
| Measure | Description | Time Frame |
|---|---|---|
| embryo euploid rate | number of euploid blastocysts divided by all blastocysts detected | about one month after blastocyst biopsy |
| embryo implantation rate | number of gestational sacs divided by number of embryo transferred |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Shuoping Zhang, Doc | Contact | +86-731-88395640 | baibaipang@hotmail.com | |
| Lu Tan, Mrs | Contact | +86-731-82355100 | 10931212@qq.com |
| Name | Affiliation | Role |
|---|---|---|
| Xiaojuan Wang, Doc | Reproductive and Genetic Hospital of Citic-xiangya | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Reproductive and Genetic Hospital of Citic-xiangya | Recruiting | Changsha | Hunan | 410000 | China |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D046149 | Embryo Culture Techniques |
| ID | Term |
|---|---|
| D046508 | Culture Techniques |
| D066298 | In Vitro Techniques |
| D008919 | Investigative Techniques |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
|
| about three to six months after blastocyst biopsy |