The Effect of Smoking and Non-surgical Periodontal Treatment on Gingival Crevicular Fluid Cyclophilin A and EMMPRIN Levels

The Effect of Smoking and Non-surgical Periodontal Treatment on Gingival Crevicular Fluid Cyclophilin A and EMMPRIN Levels

This study aimed to investigate the effect of smoking and non-surgical periodontal treatment on gingival crevicular fluid CyPA and EMMPRIN levels in patients with periodontitis. Twenty-eight patients with periodontitis (14 non-smoker and 14 smoker) and 30 control subjects (15 non-smoker and 15 smoker) were included into the study. Non-surgical periodontal treatment was applied to all periodontitis patients. Periodontal parameters and GCF CyPA and EMMPRIN levels were evaluated during 3 months following non-surgical periodontal treatment. GCF CyPA and EMMPRIN levels were analyzed by ELISA.

Inclusion Criteria:

• generalized periodontitis with stage III-IV & Grade B
• no systemic and periodontal diseases
• smokers (minimum 10 cigarettes per day for at least ten years)
• non-smokers (never smoked)

Exclusion Criteria:

• Systemic diseases
• Infectious diseases
• Pregnancy or lactating
• Having periodontal treatment during the last 6 months
• Having antibiotic treatment within the past 3 months
• Former smokers