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| Name | Class |
|---|---|
| Science and Technology Development Fund (STDF) | UNKNOWN |
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Viral myocarditis has been recognized as a cause of congestive heart failure, however diagnosis and treatment represents a challenging process. Recently, there is an increasing frequency of different cardiotropic viruses in the clinical setting of myocarditis. The introduction of the new molecular techniques in analysing the etiologic agent of acute myocarditis has enhanced significantly the knowledge on the molecular epidemiology of these viruses. The etiology of patients admitted to our university hospital remains unclear. It is therefore important to identify the aetiology associated with myocardial infections.
The purpose of the present study is to analyze the prevalence of a broad spectrum of cardiotropic viruses, including enteroviruses, adenoviruses and parvo B19 virus, in adults with suspected myocarditis with special reference to B19 virus due to its increasing prevalence nowadays.
The results of this study will provide a very important information for the prevalent infectious viral agents in our university hospital which will guide treatment protocol.
Objectives
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| patients | Experimental | all patients with myocarditis |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| endomyocardial biopsy | Diagnostic Test | Endomyocardial biopsies will be stained with Masson's trichrome as well as Giemsa and examined by light microscopy. For immunohistology, tissue sections will be treated with an avidin-biotinimmunoperoxidase method (Vectastain-Elite ABC Kit, Vector, Burlingame, Calif), with application of the following monoclonal antibodies: CD3 (T cells; Novocastra Laboratories, Newcastle, UK), CD68 (macrophages, natural killer cells; DAKO, Hamburg, Germany), and HLA-DR-α (DAKO, Hamburg, Germany |
| Measure | Description | Time Frame |
|---|---|---|
| prevalence of the most common viruses | our main interest is to determine the prevalence of the most common viruses in clinically relevant (i.e., hospitalization- requiring) patients with acute or chronic myocarditis, using highly sensitive molecular techniques as the diagnostic tool. | 12 months |
| Measure | Description | Time Frame |
|---|---|---|
| Determine the distribution of mono- and co-infections between these viral agents. | Using PCR techniques to detect if single infection or multiple viral infection per pateint | 12 months |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Assiut University Hospitals | Asyut | 71526 | Egypt |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 29622952 | Background | Hassan AKM, Fouad DA, Refaiy A. Demographic features and prevalence of myocarditis in patients undergoing transarterial endomyocardial biopsy for unexplained cardiomyopathy. Egypt Heart J. 2017 Mar;69(1):29-35. doi: 10.1016/j.ehj.2016.09.005. Epub 2016 Oct 10. | |
| 36068503 | Derived | Khidr SS, El-Mokhtar MA, Asaad SR, Hetta HF, Abdel-Rahim MH, Youssef AAA, Hassan AKM. Clinical course, viral etiology, and the diagnostic workup for patients with suspected myocarditis: a single-center prospective study. BMC Cardiovasc Disord. 2022 Sep 6;22(1):396. doi: 10.1186/s12872-022-02833-0. |
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Prospective study
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