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Obstructive sleep apnea (OSA) syndrome is associated with increased vascular dysfunction and atherosclerosis. Especially, it has been shown that OSA associated intermittent hypoxia represents a pro inflammatory stimulus resulting in macrophage polarization.
Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin signaling pathways involved in atherosclerosis. It has been shown that myeloid PTP1B deficiency protects against atherosclerosis.
As hypoxia has also been shown to increase PTP1B expression and activity, this study will evaluate the myeloid PTP1B expression and activity in patients with OSA as compared to controls and will investigate myeloid PTP1B involvement in the vascular pro inflammatory precess described in OSA.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| OSA patients | Other | 30 patients investigated for suspected OSA with an apnea hypopnea index ≥ 15 per hour |
|
| non OSA patients | Other | 30 patients investigated for suspected OSA with an apnea hypopnea index < 15 per hour |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| myeloid PTP1B expression analysis | Other | peripheral blood macrophage PTP1B expression |
|
| Measure | Description | Time Frame |
|---|---|---|
| myeloid PTP1B expression | Peripheral blood will be collected from OSA and non OSA patients. Macrophage will be isolated by specific centrifugation. PTP1B expression will be assessed by Western Blot. | baseline |
| myeloid PTP1B activity | Peripheral blood will be collected from OSA and non OSA patients. Macrophage will be isolated by specific centrifugation. PTP1B activity will be assessed by specific colorimetric assay. | baseline |
| Measure | Description | Time Frame |
|---|---|---|
| PTP1B exosomal expression. | Peripheral blood will be collected from OSA and non OSA patients. Exosomes will be isolated by consecutive centrifugations. PTP1B expression will be assessed by Western Blot. | baseline |
| in vitro PTP1B implication on macrophage inflammatory response |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Wojciech Trzepizur, MD Ph D | University Hospital, Angers | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Angers University Hospital | Angers | 49100 | France |
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| ID | Term |
|---|---|
| D012891 | Sleep Apnea Syndromes |
| D007249 | Inflammation |
| D050197 | Atherosclerosis |
| ID | Term |
|---|---|
| D001049 | Apnea |
| D012120 | Respiration Disorders |
| D012140 | Respiratory Tract Diseases |
| D020919 | Sleep Disorders, Intrinsic |
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patients with OSA 'apnea hypopnea index ≥ 15 per hour) as compared to controls (an apnea hypopnea index < 15 per hour)
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Macrophage isolated from patients will be stimulated by pro inflammatory agents (IL6, TNF alpha) in the presence and absence of PTP1B inhibitor (MSI-1436). The inflammatory response will be assessed by measuring inflammatory cytokines in the supernatant by ELISA. |
| baseline |
| D020920 |
| Dyssomnias |
| D012893 | Sleep Wake Disorders |
| D009422 | Nervous System Diseases |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D001161 | Arteriosclerosis |
| D001157 | Arterial Occlusive Diseases |
| D014652 | Vascular Diseases |
| D002318 | Cardiovascular Diseases |