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| Name | Class |
|---|---|
| American Egg Board | OTHER |
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The main objective of the study is to demonstrate that the inclusion of eggs to a plant-based diet will not increase the risk for heart disease but will increase the concentration of lutein and zeaxanthin, two important antioxidants and will also increase choline, an important component of membrane phospholipids.
Results from studies involving cells, animal models and clinical trials have demonstrated that lutein and zeaxanthin potentially protect against chronic diseases, including age-related macular degeneration, cataracts, coronary heart disease and stroke. Plasma concentrations of lutein and zeaxanthin are closely associated with their antioxidant properties and with their protection against reactive oxygen species (ROS).
Choline is recognized as an essential nutrient for health because of its many functions in growth and development, as well as neurological function and formation of membrane phospholipids, including phosphatidylcholine (PC) and sphingomyelin.
Therefore the objective of this study is to demonstrate that inclusion of eggs in a plant-based diet will potentiate the benefits of this healthy diet by increasing plasma concentrations of lutein, zexanthin and choline presents in egg yolks and theoretically increase the antioxidant and anti-inflammatory actions of these components. This would be potentially more important in those individuals with metabolic syndrome, which is the target population of this intervention.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Egg intake | Experimental | Consumption of 2 eggs with spinach daily for breakfast for 4 weeks |
|
| Egg Subsitute | Experimental | Consumption of 2 egg substitutes daily for breakfast for 4 weeks |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Consume 2 eggs with spinach per day | Other | Participants will be randomly allocated to consume either 2 eggs or 2 egg substitutes with spinach daily for 4 weeks and after a 3-week wash out, they will be allocated to the alternate treatment |
| Measure | Description | Time Frame |
|---|---|---|
| Plasma lutein | Plasma lutien will be measured by HPLC. The units are expresses as µmol/L | 1 year |
| Plasma choline | It will be measured by mass spectometry. The units are expressed as nmol/mL | 1 year |
| Plasma Zeaxanthin | Plasma zeaxanthin will by measured by HPLC. The units are expressed as µmol/L | 1 year |
| Measure | Description | Time Frame |
|---|---|---|
| Plasma lipids | Total, LDL and HDL cholesterol and triglycerides will be measured by an automated spectrometer simultaneously. The units are expressed as mg/dL | 1 year |
| Plasma glucose | Plasma glucose will be measured by an automated spectrometer. Units are expressed as mg/dL |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Maria-Luz Fernandez, PhD | University of Connecticut | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Nutritional Sciences | Storrs | Connecticut | 06269 | United States |
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| ID | Term |
|---|---|
| D003324 | Coronary Artery Disease |
| D003920 | Diabetes Mellitus |
| D024821 | Metabolic Syndrome |
| ID | Term |
|---|---|
| D003327 | Coronary Disease |
| D017202 | Myocardial Ischemia |
| D006331 | Heart Diseases |
| D002318 | Cardiovascular Diseases |
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Randomized Crossover design
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Egg or Egg substitutes (whites only) will be provided to subjects and since they are similar in appearance and color, they will not know when they are consuming them
|
| Egg Subsitute with spinach per day | Other | Egg Subsitute with spinach per day |
|
| 1 Year |
| D001161 |
| Arteriosclerosis |
| D001157 | Arterial Occlusive Diseases |
| D014652 | Vascular Diseases |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
| D004700 | Endocrine System Diseases |
| D007333 | Insulin Resistance |
| D006946 | Hyperinsulinism |