Sperm DNA Fragmentation Effects on Blastocyst Morphology Grading, Pregnancy Rate and Implantation Rate

The Correlation of Sperm DNA Fragmentation With Blastocyst Grading and Its Implantation Potential

Retrospective cohort study for 189 couples assigned to normal and abnormal Sperm DNA fragmentation (SDF) groups.To determine the effect of SDF on blastocyst's trophectoderm (TE), Inner cell mass (ICM) morphology grading, and implantation rate. Semen in both groups was processed by density gradient (DG).

Purpose: to determine if sperm DNA fragmentation (SDF) has a correlation with Blastocyst's trophectoderm (TE) and Inner cell mass (ICM) grading, or with the implantation rate?

Method: Retrospective cohort study for 189 couples, assigned to normal SDF group (121 cases), and abnormal SDF group (68 cases). Semen in both groups was processed by double layer density gradient (DG).

Statistical analysis done using SPSS version 22.

Inclusion Criteria:

• All cases in this study have to reach the blastocyst stage.
• Female age ≤37 years old.
• Male sperm count ≥ 5 million.

Exclusion Criteria:

• Usage of sperm selection techniques.
• Testicular aspiration or testicular biopsies.
• Oocyte or sperm donation.