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Retrospective cohort study for 189 couples assigned to normal and abnormal Sperm DNA fragmentation (SDF) groups.To determine the effect of SDF on blastocyst's trophectoderm (TE), Inner cell mass (ICM) morphology grading, and implantation rate. Semen in both groups was processed by density gradient (DG).
Purpose: to determine if sperm DNA fragmentation (SDF) has a correlation with Blastocyst's trophectoderm (TE) and Inner cell mass (ICM) grading, or with the implantation rate?
Method: Retrospective cohort study for 189 couples, assigned to normal SDF group (121 cases), and abnormal SDF group (68 cases). Semen in both groups was processed by double layer density gradient (DG).
Statistical analysis done using SPSS version 22.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Normal SDF | Couples with male partners having SDF lower than 20% using TUNEL assay |
| |
| Abnormal SDF | Couples with male partners having SDF greater than 20% using TUNEL assay |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| ICSI | Procedure | Intracytoplasmic sperm injection of female partner oocytes with ejaculated sperm selected by density gradient centrifugation |
|
| Measure | Description | Time Frame |
|---|---|---|
| Blastocyst morphological grading | Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A to C ( A is the highest quality). | 5-6 days after ICSI |
| Implantation rate | Percentage of the implanted embryos number for each group / number of transferred embryos. | 6 weeks of pregnancy |
| Measure | Description | Time Frame |
|---|---|---|
| Fertilization rate | Percentage of fertilized oocyte (appearance of two pronuclei) / Number of injected oocytes | 16-19 hours of ICSI |
| Blastulation rate | Percentage of total blastocysts / total 2PN |
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Inclusion Criteria:
Exclusion Criteria:
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female age ≤37 years old, with no limitation on male age
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ICSI patients in Ganin Fertility Center from January 2018 to September 2018.
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| Name | Affiliation | Role |
|---|---|---|
| Manar Hozyen, MSc | Ganin Fertility Center | Principal Investigator |
| Eman Hassanen, BSc | Ganin Fertility Center | Principal Investigator |
| Hosam Zaki, MSc, FRCOG | Ganin Fertility Center | Study Chair |
| Hanaa El Khedr | Ganin Fertility Center | Principal Investigator |
| Yasmine Azzouz, BSc | Ganin Fertility Center | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ganin Fertility Center | Cairo | 11728 | Egypt |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 27579009 | Background | Wdowiak A, Bojar I. Relationship between pregnancy, embryo development, and sperm deoxyribonucleic acid fragmentation dynamics. Saudi J Biol Sci. 2016 Sep;23(5):598-606. doi: 10.1016/j.sjbs.2015.08.001. Epub 2015 Aug 10. | |
| 28591199 | Background | Gat I, Tang K, Quach K, Kuznyetsov V, Antes R, Filice M, Zohni K, Librach C. Sperm DNA fragmentation index does not correlate with blastocyst aneuploidy or morphological grading. PLoS One. 2017 Jun 7;12(6):e0179002. doi: 10.1371/journal.pone.0179002. eCollection 2017. |
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| ID | Term |
|---|---|
| D020554 | Sperm Injections, Intracytoplasmic |
| ID | Term |
|---|---|
| D005307 | Fertilization in Vitro |
| D027724 | Reproductive Techniques, Assisted |
| D012099 | Reproductive Techniques |
| D013812 | Therapeutics |
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semen, oocytes, blastocysts
| 5-6 days of ICSI |
| Pregnancy rate | Percentage of pregnant cases / transferred cases for each group ( positive pregnancy test) | After 2 weeks of embryo transfer |
| 29116706 | Background | Alvarez Sedo C, Bilinski M, Lorenzi D, Uriondo H, Noblia F, Longobucco V, Lagar EV, Nodar F. Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects. JBRA Assist Reprod. 2017 Dec 1;21(4):343-350. doi: 10.5935/1518-0557.20170061. |
| D008919 | Investigative Techniques |