This study was performed to evaluate the effects of both periodontal treatment and smoking on the gingival crevicular fluid(GCF) levels of interleukin (IL)-17A, IL-17E, Total oxidative stress(TOS) and total antioxidant capasity(TAOC) in smoking and non-smoking patients with severe chronic periodontitis.

Systemically healthy 15 smoker,15 non-smoker with chronic periodontitis were attended for the study. Periodontal status were determined by recording periodontal clinical measurements. GCF samples were collected at the baseline, at the end of initial periodontal treatment and 4 weeks after periodontal flap surgery. The data were tested statistically by the Student t and paired sample t tests.

Inclusion Criteria:

• individuals aged ˃35 years and having ≥16 teeth diagnosed as chronic periodontitis
• not received any periodontal therapy within the previous 6 months;
• not received antibiotic/anti-inflammatory drug treatment within the previous 6 months.

Exclusion Criteria:

• receiving medical treatment for cancer, rheumatoid arthritis, diabetes mellitus, cardiovascular disease, or any other systemic disease;
• being pregnant or being in the period of menopause or lactation;
• receiving any drug therapy that might affect the clinical characteristics of periodontitis;
• usage of systemic antimicrobials during the 6 weeks prior to data collection

Patients with chronic periodontitis (with an age range of 35 to 60 years) seeking dental treatment at the Periodontology Clinic, School of Dentistry, Ondokuz Mayis University, were included in the present study.