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| Name | Class |
|---|---|
| York St John University | OTHER |
| Newcastle University | OTHER |
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This study involves drawing blood samples from men before and after they perform 30-minutes of moderate-intensity aerobic exercise. The investigators will evaluate whether adding the exercise serum to colon cancer cells in a dish can reduce the growth of the cells compared to the resting serum.
Note: serum is the liquid part of the blood that carries hormones and metabolites around the body.
Regular exercise is associated with a reduced risk of developing colon cancer. However, the mechanisms underpinning the anti-cancer effect of exercise are not yet fully understood. A recent theory suggests that each time you exercise, the short-lasting spikes in circulating hormones can suppress the growth of cancer cells. Hence, every exercise bout could have a direct anti-cancer effect.
This study will recruit men with an increased of colon cancer and explore whether incubating colon cancer cells with serum collected after a bout of moderate-intensity aerobic exercise influences cell viability in vitro.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Exercise assessment | Experimental | The exercise condition will involve venous blood samples being drawn immediately before and after a single bout of moderate-intensity aerobic interval exercise. |
|
| Resting assessment | No Intervention | The resting condition will involve venous blood samples being drawn before and after 60 minutes of seated rest. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Exercise assessment | Other | The moderate-intensity aerobic interval exercise will be performed on a cycle ergometer under the supervision of trained staff in an exercise science laboratory. Participants will perform a 5 to 10-minute warm-up that begins by pedalling against a light resistance (60 W) and progressively increases in resistance until a target heart rate of 50-60% heart rate reserve is achieved. Participants will then complete 6 x 5-minute bouts at 60% heart rate reserve whilst maintaining a cadence of 60 rev·min-1, separated by 2.5-minutes of pedalling against light resistance (60 W). The session will finish with a cool-down at light resistance (60 W) lasting 10-minutes. |
| Measure | Description | Time Frame |
|---|---|---|
| Cell viability (%) | Viability of a human colon cancer cell line (LoVo) will be assessed via quantification of the fluorescent signal by the resazurin assay (Sigma-Aldrich, Dorset, UK). Cells will be seeded in culture medium containing 10% serum from individual participants and incubated for 48 hours. Fluorescence will then be measured using a microplate reader at an excitation of 540 nm and emission of 590 nm. Background fluorescence will be subtracted from each well and then values will be normalised to fluorescence of control cells grown in 10% fetal bovine serum instead of participant serum to give a percent viability. | 48 hours |
| Measure | Description | Time Frame |
|---|---|---|
| Concentration of epinephrine (pg/ml) | Systemic concentration of epinephrine will be measured from serum | Immediately before and immediately after the exercise and resting assessments |
| Concentration of norepinephrine (pg/ml) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Samuel T Orange, PhD | Northumbria University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| York St John University Sports Park | York | North Yorkshire | YO31 8FY | United Kingdom | ||
| Northumbria University City Campus |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 22914790 | Background | Boyle T, Keegel T, Bull F, Heyworth J, Fritschi L. Physical activity and risks of proximal and distal colon cancers: a systematic review and meta-analysis. J Natl Cancer Inst. 2012 Oct 17;104(20):1548-61. doi: 10.1093/jnci/djs354. Epub 2012 Aug 22. | |
| 28887324 | Background | Dethlefsen C, Hansen LS, Lillelund C, Andersen C, Gehl J, Christensen JF, Pedersen BK, Hojman P. Exercise-Induced Catecholamines Activate the Hippo Tumor Suppressor Pathway to Reduce Risks of Breast Cancer Development. Cancer Res. 2017 Sep 15;77(18):4894-4904. doi: 10.1158/0008-5472.CAN-16-3125. |
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Raw data and statistical code will be uploaded onto a publicly available repository
Raw data will be made available immediately after publication of the aggregated data in a peer-reviewed Journal
Publicly available
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| ID | Term |
|---|---|
| D003110 | Colonic Neoplasms |
| ID | Term |
|---|---|
| D015179 | Colorectal Neoplasms |
| D007414 | Intestinal Neoplasms |
| D005770 | Gastrointestinal Neoplasms |
| D004067 | Digestive System Neoplasms |
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This is a randomised, counterbalanced, crossover study whereby all participants will complete an exercise assessment and a resting assessment in a randomised order.
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It is not possible to blind participants nor investigators. Investigators will be blind to allocation order until after the first blood sample is drawn.
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|
Systemic concentration of norepinephrine will be measured from serum
| Immediately before and immediately after the exercise and resting assessments |
| Concentration of Interleukin 6 (pg/ml) | Systemic concentration of Interleukin 6 will be measured from serum | Immediately before and immediately after the exercise and resting assessments |
| Concentration of Tumour Necrosis Factor alpha (pg/ml) | Systemic concentration of Tumour Necrosis Factor alpha will be measured from serum | Immediately before and immediately after the exercise and resting assessments |
| Concentration of Insulin (pmol/l) | Systemic concentration of Insulin will be measured from serum | Immediately before and immediately after the exercise and resting assessments |
| Concentration of Insulin-like growth factor 1 (ng/ml) | Systemic concentration of Insulin-like growth factor 1 will be measured from serum | Immediately before and immediately after the exercise and resting assessments |
| Newcastle upon Tyne |
| NE1 8SG |
| United Kingdom |
| 28138894 | Background | Dethlefsen C, Pedersen KS, Hojman P. Every exercise bout matters: linking systemic exercise responses to breast cancer control. Breast Cancer Res Treat. 2017 Apr;162(3):399-408. doi: 10.1007/s10549-017-4129-4. Epub 2017 Jan 30. |
| 30812059 | Background | Devin JL, Hill MM, Mourtzakis M, Quadrilatero J, Jenkins DG, Skinner TL. Acute high intensity interval exercise reduces colon cancer cell growth. J Physiol. 2019 Apr;597(8):2177-2184. doi: 10.1113/JP277648. Epub 2019 Mar 20. |
| D009371 | Neoplasms by Site |
| D009369 | Neoplasms |
| D004066 | Digestive System Diseases |
| D005767 | Gastrointestinal Diseases |
| D003108 | Colonic Diseases |
| D007410 | Intestinal Diseases |