This investigations examined the effect that posture has on metabolic and cardiovascular measurements. Further, it will explore the potential for body weight status or physical activity to influence these relationships. All subjects will complete a seated condition and a standing condition in a counterbalanced manner.

The purpose of the present study was to evaluate both metabolic and cardiovascular responses to acute bouts of computer work while sitting and standing. Cardiovascular measurements were made using continuous non-invasive blood pressure monitoring and metabolic measurements were made using indirect calorimetry. Subjects will be grouped and analyzed based upon the weight status of the subject. All subjects will complete a seated condition and a standing condition in a counterbalanced manner. Investigators hypothesize that metabolic and cardiovascular responses will be elevated in the standing condition compared to sitting.

Inclusion Criteria:

• healthy adult

Exclusion Criteria:

• Have a history of cancer, cardiac issues, hypertension, pulmonary disease, stroke, neurological or muscular dystrophy, uncontrolled diabetes, or any life threatening chronic conditions.
• Are currently being treated for infectious mononucleosis, hepatitis, pneumonia, or other infectious diseases.
• Are unable or unwilling to stand in one position continuously for 15-20 minutes at a time.
• Use nicotine products, or non-contraceptive hormonal therapy (birth control is okay).
• Are pregnant or trying to become pregnant.
• Are not between 18 and 50 years of age.

Healthy subjects between the ages of 18 and 50 years.